Analytical Chemistry Unit 3

Gel Filtration

Hydrophilic stationary phase with an aqueous eluent

Gel Permeation

Hydrophobic stationary phase with an organic eluent

Irradiance (P)

Energy per second per unit area of light beam

Monochromator

A device (such as a prism) that disperses light into its component wavelengths and then only allows light of one of those specific wavelengths to pass through its exit slit.

Transmittance

The fraction of original light that passes through a sample

Absorbance

-log(T) Directly proportional to concentration

Molar Absorption Coefficient

A constant of proportionality that describes how much light is absorbed at a particular wavelength for a particular substance.

Absorbance Spectrum

A graph of how absorbance or molar absorptivity coefficient varies with wavelength

Chromophore

The part of a molecule that is responsible for absorbing light

Reagent Blank

Contains all the reagents except for the analyte which has been replaced with distilled water