Culture Media

Contains: Types of Culture Media, Routine Media for Aerobes and Facultative Anaerobes, Selective Media for Neissaria gonorrhae and meningitidis, and Special Bacteriologic Media

What are the 4 types of culture media? (SEND)

Selective

Enrichment

Nutritive

Differential

Describe Selective Media…

A media that contains additives such as dyes, bile salts, alcohols, acids, or antibiotics to inhibit growth of certain bacteria

Describe Enrichment Media

A media that contains added growth factors

Why do we call it “Nutritive media”?

Because it supports the growth of MOST nonfastidious bacteria

Nonfastidious vs. Fastidious bacteria

N: Does not require special conditions for growth

F: Requires special nutritional supplements or conditions for growth

Describe Differential Media

A media that contains compounds that allow certain bacteria (or even species) to be visually differentiated

What are some methods of Differential Media?

Lactose fermentation and Hydrogen sulfide production (H₂S)