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What is Replicative Life Span (RLS) in yeast?
The number of mitotic divisions a mother cell undergoes before senescence.
What limits RLS in yeast?
Accumulation of Extrachromosomal rDNA Circles (ERCs).
How is RLS measured?
Via micromanipulation (every ~90 minutes).
What human cell type does RLS model?
Mitotically active cells (e.g., stem cells).
What is Chronological Life Span (CLS) in yeast?
The length of time non-dividing cells remain viable in stationary (G0) phase.
How is CLS measured?
Via CFUs or Phloxin B staining.
What human cell type does CLS model?
Post-mitotic cells (e.g., neuron