Bio Lab 11

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Last updated 4:36 AM on 6/24/26
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9 Terms

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plasmid transformation in nature

plasmid tranferred between bacteria - conjuction

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plasmid transformation in the lab - recombinant DNA

DNA put into plasmid, plasmid put into desired cell

re-combine DNA from different organism

ex. human gene into bacteria, gene from one plant into another

3
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restriction enzymes

“scissors”

cut DNA

made by bacteria to fight viruses (bacteriophage), cut at palindromes leaving sticky ends

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Ligase enzymes

“glue”

attach DNA

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araC

bifunction regulatory protein

represses transcription/stops synthesis of green fluorescent protein

unless bound to arabinose - used to turn on the glow (allows GFP to be made)

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Psad

arabinose prompoter

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GFP

green Fluorescent Protein gene

protein gene

under control of Pbad

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bla

beta-lactamase gene - ampicillin resistance

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araC gene —> araC protein

gene binds and represses transcription —> protein binds to arabinose now it cannot repress transcription (arabinose has repressed the repressor)