Micro Lab 7

Handwashing is an example of ______

degerming

Wiping the skin with an alcohol swab at an injection site is an example of ________

degerming

What are the other two names for Kirby-Bauer method

disk diffusion test and antibiotic susceptibility test

Kirby-Bauer method

most used method to determine is an organism if susceptible or resistant to a selection of antimicrobial agents. It can also be used to test the efficacy of a new antimicrobial

What type of agar plate is used for Kirby-Bauer

Mueller-Hinton

Which factors affect the outcome of the KB test

rate at which each drug diffuses, incubation time, concentration of inoculum

What is the “bacteria-free” zone called around a disk

zone of inhibition

What unit is the zone of inhibition measured in

mm (millimeters)

What do you absolutely need when determining a conclusion for KB

standard zone diameter interpretive chart

What are the three results your antibiotic can receive (RSI)

resistant, susceptible, intermediate

Broad spectrum antibiotics

active against a wide range of bacteria

Narrow spectrum antibiotics

limited activity and active against particular species

If you know the pathogen of the infection, which drug would you use?

narrow spectrum

If you don’t know the pathogen of the infection, which drug would you use?

broad spectrum

Reasons why one would use broad spectrum

infection is unknown (but its bacterial), infection with multiple groups, when a narrow drug fails due to drug resistance

Antibiogram

a compilation of local antibiotic susceptibility data for several bacterial strains