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Obligate aerobe
E requires oxygen for growth
Microaerophile
D requires small amount of oxygen
Facultative anaerobe
B grows with or without oxygen
Aerotolerant anaerobe
F tolerates oxygen but does not use it
Obligate anaerobe
C grows only without oxygen
Capnophile
A requires increased CO2
Hydrogen peroxide
A degraded by catalase
Superoxide anion
C toxic oxygen radical
Hydroxyl radical
B most potent oxidant
Superoxide dismutase
C converts superoxide radicals
Catalase
E breaks down hydrogen peroxide
Thioglycollate
A reducing agent for anaerobic media
Cysteine
B reducing agent added to media
Dithiothreitol
C maintains low redox potential
C. botulinum
C botulism
C. tetani
D tetanus (lockjaw)
C. difficile
A pseudomembranous colitis
C. perfringens
B gas gangrene
C. septicum
E marker for GI malignancy
Botulinum toxin
D prevents acetylcholine release
Tetanospasmin
C prevents neurotransmitter release
α-toxin
A causes tissue necrosis
Toxin A
B enterotoxin
Toxin B
E cytotoxin
Trismus
C lockjaw
Risus sardonicus
A distorted grin
Pseudomembranous colitis
B bloody diarrhea
Bullae
D gas gangrene finding
Sulfur granules
E actinomycosis
Actinomyces israelii
B branching organism
Lactobacillus
A largest vaginal flora
Mobiluncus
C bacterial vaginosis
Propionibacterium
D blood culture contaminant
Bifidobacterium
E forked morphology
Bacteroides fragilis
B most common anaerobic isolate
Prevotella
D does not grow on BBE
Porphyromonas
C brick-red fluorescence
Fusobacterium
A fusiform rods
Bilophila wadsworthia
E fish-eye appearance
BBE agar
C Bacteroides growth
KVLB agar
D Prevotella growth
CCFA
A C. difficile isolation
CHOC
E aerobic with CO2
EYA
B lecithinase/lipase detection
Aspirate
B needle and syringe collection
Swab
A unacceptable for anaerobes
Tissue biopsy
C maintains reduced atmosphere
PRAS medium
D oxygen-free transport
Thioglycollate broth
E used for swab transport
C. perfringens
A gas gangrene
C. tetani
B lockjaw
C. botulinum
C botulism
C. difficile
D pseudomembranous colitis
C. septicum
E GI malignancy marker
Hydroxyl radical
B most toxic oxygen product
Hydrogen peroxide
A substrate for catalase
Superoxide dismutase
C converts superoxide
Singlet oxygen
D reactive oxygen species
Clostridium tetani
A terminal spore
Clostridium septicum
B subterminal spore
C. perfringens
C most common gas gangrene cause
C. difficile
D barnyard odor
Actinomyces
E molar tooth colonies
Bifidobacterium
A forked morphology
Propionibacterium
D diphtheroid contaminant
Eggerthella
C small circular colonies
Finegoldia magna
C most pathogenic anaerobic coccus
Veillonella parvula
B gram-negative anaerobic coccus
Peptostreptococcus
A anaerobic cocci group
Anaerococcus
D urease positive species
Hydroxyl radical
B most potent oxidant
Hydrogen peroxide
A toxic oxygen product
Superoxide anion
C reactive oxygen species
Lockjaw
D tetanus
Gas gangrene
B myonecrosis
Pseudomembranous colitis
A C. difficile disease
Actinomycosis
C sulfur granules disease
BBE agar
C Bacteroides growth medium
KVLB agar
D Prevotella medium
CCFA
A C. difficile medium