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molecular markers
are genes or DNA sequences controlling traits within chromosomal locations in organisms
Types of molecular markers
Rna based
Dna sequencing
Fingerprinting
Dna and gene family dependant markers
endonuclease/restriction base
Applications of molecular markers:
Screening population of plants to identify the individuals with genes of interest at the early stage of development
MPS (massively parallel sequencing) allows for the identification of an individual including age, eye color, ancestry and species
Detection of mutants for disease resistance
Phylogenetics for molecular evolution studies
DNA barcoding for species identification
Paternity testing/DNA fingerprinting
Mendelian inheritance (co dominant or dominant)
Bioinformatics:
Bioinformatics is the discipline of quantitative analysis of information relating to biological macromolecules with the aid of computers.
Compositions of bioinformatics:
Computer science
Mathematics and statistics
Biology
Branches of bioinformatics: 4 of them
Genomics (the genome at DNA level)
Proteomics (the entire protein make up or repertoire of a genome)
Transcriptomics (expression of genes, cDNA)
Structural genomics (determination of molecular structure via protein in genomic sequence analysis)
Aims of bioinformatics:
Organizes data for researchers to access existing information and to submit new entries
Develops tools and resources that enhance the analyses of data
Uses the tools to analyze data and interpret the results in a biologically meaningful manner
Types of bioinformatics databases:
GenBank (nucleotide sequences)
Ensembl (human/mouse genome)
Pubmed(literature references)
Enzymes (enzymes)
PDB(protein structures)
OMIM (genetic sequences)
KEGG (metabolic pathways)
NR (protein sequences)
SWISS-PROT (protein sequences)
InterPro (protein domain)
tools of bioinformatics as well as collection and retrieval of data.
Blast nucleotide sequences
Expasy for protein analysis
MEGA4
DnaSP6
CLC
Codoncode aligner