AUBF - FECAL - CHEMICAL TESTING

quanti, APT, Carbs

VAN DE KAMER TITRATION

Fecal lipids are converted to fatty acids and titrated to a neutral endpoint with sodium hydroxide

Gravimetric method

detects all fecal fats

HYDROGEN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Rapid (5 minutes) and safe procedure for analyzing quantitative fecal fat

HYDROGEN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Normal values based on 100g/day intake of fat: 1-6 gram per day or a coefficient of fat retention of at least 95%

ACID STEATORIT

Rapid test to estimate the amount of fat excretion

ACID STEATORIT

Monitor a patient's response to therapy and screen for steatorrhea in pediatric populations

ACID STEATORIT

measures upper layer of fat overlying solid fecal layer

NEAR INFRARED REFLECTANCE SPECTROSCOPY

Rapid procedure for fecal fat that requires less stool handling by laboratory personnel

NEAR INFRARED REFLECTANCE SPECTROSCOPY

Does not require reagents after homogenization of the specimen

APT TEST FOR FETAL HEMOGLOBIN

Distinguishing between the presence of fetal blood or maternal blood in an infant's stool

Pancreatic enzymes

essential for digesting dietary proteins, carbohydrates, and fats

pancreatic insufficiency

Associated with disorders such as chronic

pancreatitis and cystic fibrosis

trypsin

If __ is present = it digests the gelatin on the paper, leaving a clear area

CHYMOTRYPSIN

More resistant to intestinal degradation than trypsin

CHYMOTRYPSIN

More sensitive indicator of less severe cases of pancreatic insufficiency

ELASTASE I

Pancreas specific (enzyme produced solely by pancreas)

ELASTASE I

strongly resistant to degradation

ELASTASE I

absence strongly indicate that it was not produced by the pancreas in the first place

ELASTASE I

Very sensitive indicator of exocrine pancreatic insufficiency

CARBOHYDRATES

Detected by performing a copper reduction test on the fecal specimen