Lab 10 micro

Which of the following is the most widely used molecular tool in biology?

PCR

Which of the following is the most widely used bioinformatic tool in biology?

BLAST

Which genetic locus is typically used to identify bacteria and archaea?

16s rRNA

Which two genetic loci is typically used to identify fungi and other eukaryotes?

COI
ITS

In DNA extraction, the first step is to

Break the cells open to expose the DNA

In DNA extraction, the second step is to

Remove lipids and proteins

In DNA extraction, the third step is to

Precipitate the DNA with alcohol

Which reagent of PCR stabilizes the molecular reactions?

Buffer

Which reagent of PCR creates covalent bonds to make the new DNA strand?

Taq polymerase

Which reagent of PCR hybridizes with the DNA at the location where amplification will occur?

Primers

Which reagent of PCR is the building blocks of the new DNA?

dNTP's

Which type of PCR is used to quantify how much RNA is being produced?

Reverse transcriptase PCR

Which type of PCR is used to amplify large amount of a DNA target and then sequenced?

Conventional PCR

Which type of PCR is used as a diagnostic to distinguish between different microbes?

Real-time PCR

Which phase of PCR allows for the primers to form hydrogen bonds with the single stranded DNA?

Annealing

Which phase of PCR raises the temperature to break the DNA into single strands?

Denaturation

Which phase of PCR uses the Taq polymerase to add complementary nucleotides to the single strand of DNA?

Extension

Which phase of PCR has an optimal temperature at 72 degrees Celsius?

Extension

Which phase of PCR has an optimal temperature at 95 degrees Celsius?

Denaturation

Which phase of PCR has an optimal temperature at 50-60 degrees Celsius?

Annealing

This BLAST result is used as a comparison between searches.

Max or total score

This BLAST result illustrates how much of the inputted sequence is used in the alignment.

Percent query coverage

This BLAST result is the number of alignments that occur by chance.

Expect value

This BLAST result illustrates how well the inputted sequence matches to sequences in the database.

Percent similarity

When using a database to identify a microbe, which is the preferred result to use?

Percent Similarity