Chapter 2-Part 1: Intestinal, Commensal, and Free-living Pathogenic Amebae

This is the first part of Chapter 2. Study well.

Species Name:
Group:
Pointed in Arrow:
Stage:
Life Cycle Stages of this species:
How many nuclei does it have:

Species Name: E. histolytica
Group: Amoeba (Sarcodina)
Pointed in Arrow: Chromatoid body
Stage: Cyst
Life Cycle Stages of this species: Cyst and Trophozoite
How many nuclei does it have: 4

Species Name:
Pointed in Arrow:
Stage:
Infective and Invasive stage:
Primary Host and Reservoir:

Species Name: E. histolytica
Pointed in Arrow: Chromatoid body
Stage: Cyst
Infective and Invasive stage: Cyst and Trophozoite
Primary Host and Reservoir: Humans

7 Species of Intestinal Amebae in Humans

1. Pathogenic:

2. Commensals:

7 Species of Intestinal Amebae in Humans

1. Pathogenic: Entamoeba histolytica

2. Commensals: E. dispar, E. moshkovskii, E. hartmanni. E. coli, Endolimax nana, Iodamoeba butschlii
   Mnemonic: (HMDCNB - Hugaw Man Diay Ca Na Bata)

Species and what stage:
Infective Stage:
Diagnostic Stage:
Pathognomonic finding:
MOT:
Lab Diagnosis:
Pathogenicity:
Treatment:
Red Line:
Yellow Line:

Species: E. histolytica, Trophozoite
Infective Stage: Mature Quadrinucleate Cyst
Diagnostic Stage: Cyst and Trophozoite in Stool
Pathognomonic finding: Trophozoite with ingested RBCs
MOT: Fecal-Oral Route, Mechanical Vectors, Sexual contact
Lab Diagnosis: Stool Microscopy, ELISA, PCR, Isoenzyme analysis
Pathogenicity: Amoebic colitis, Amoebic Liver Abscess (ALA)
Treatment: Metronidazole, Tinidazole
Red Line: Nucleus
Yellow Line: Ingested RBC

Species and Stage:
Locomotion:

Species and Stage: E. histolytica, Trophozoite
Locomotion: Pseudopodia

What type of ulcer is this?
What parasite is the cause?

Flask-shaped Ulcer
E. histolytica

1. What is this?

2. If you have this, you suffer from?

3. What species is responsible for this?

4. Drug of choice. Give the alternative DOC.

1. Charcot-Leyden Crystal

2. Amebiasis

3. E. histolytica

4. Metronidazole. Tinidazole, Secnidazole

1. Species

2. Describe it’s survival ability.

3. What species is it morphologically identical with?

1. Entamoeba moshkovskii

2. Osmotolerant, Survives 0-41 C

3. E. histolytica

1. Species

2. Stage

3. Movement

4. Number of Nuclei

1. Entamoeba coli

2. Cyst

3. Sluggish Movement

4. 8

1. Species

2. Stage

3. Movement

4. Number of Nuclei

1. Entamoeba coli

2. Cyst

3. Sluggish Movement

4. 8

1. Species

2. Stage

1. Entamoeba coli

2. Trophozoite

1. Species

2. Also known as:

3. Stage

1. Entamoeba hartmanni

2. Small race of E. histolytica

3. Cyst

1. Species

2. Stage

1. Entamoeba hartmanni

2. Trophozoite

1. Species

2. Stage

1. Entamoeba hartmanni

2. Trophozoite

1. Species

2. Stage

1. Entamoeba polecki

2. Cyst

1. Species

2. Stage

1. Entamoeba polecki

2. Cyst

1. Species

2. Stage

1. Entamoeba polecki

2. Cyst

1. Species

2. Stage

3. Red:

4. Yellow:

1. Entamoeba polecki

2. Cyst

3. Large nucleus with pleomorphic karyosome

4. Chromatoid bodies

1. Species

2. Stage

1. Entamoeba polecki

2. Trophozoite

1. Species

2. Stage

1. Entamoeba polecki

2. Trophozoite

1. Species

2. Stage

1. Entamoeba polecki

2. Trophozoite

1. Species

2. Stage

3. Distinguishing feature

4. Source of infection

1. Iodamoeba buetschlii

2. Cyst

3. Glycogen vauole

4. Contaminated hog feces

1. Species

2. Stage

3. Black Arrow

4. Sources of infection

1. Iodamoeba buetschlii

2. Cyst

3. Glycogen vauole

4. Contaminated hog feces

1. Species

2. Stage

3. Black Arrow

4. Source of infection

1. Iodamoeba buetschlii

2. Cyst

3. Glycogen vauole

4. Contaminated hog feces

1. Species

2. Stage

1. Iodamoeba buetschlii

2. Trophozoite

1. Species

2. Stage

1. Iodamoeba buetschlii

2. Trophozoite

1. Species

2. Stage

1. Iodamoeba buetschlii

2. Trophozoite

1. Species

2. Stage

1. Endolimax nana

2. Cyst

1. Species

2. Stage

1. Endolimax nana

2. Trophozoite

1. Species

2. Stage

1. Endolimax nana

2. Trophozoite

1. Species

2. Stage

1. Endolimax nana

2. Cyst

1. Species

2. Stage

3. Transmission

4. Feeds on

5. Unique Characteristic

6. Treatment

1. Entamoeba gingivalis

2. Trophozoite

3. Kissing, Droplet spray, shared utensil

4. Feeds on leukocytes and bacteria

5. Unique characteristic: No cyst stage

6. No treatment required (non-pathogenic)

1. Species

2. Stage

3. Causes what diseases:

4. Prominent Characteristic

5. Give at least two entry pathway to the body

6. This is a possible reservoir hosts form medically important bacteria such as… give at least two

1. Acanthamoeba spp.

2. Trophozoite

3. Acanthamoeba Keratitis (AK), Granulomatous Amebic Encephalitis (GAE)

4. Acanthopodia (thorn-like projections)

5. Eye, Nasal Passages, Broken skin

6. Legionella spp., mycobacteria, E. coli

1. Species

2. Stage

3. Found in… give at least two

1. Acanthamoeba spp.

2. Trophozoite

3. Freshwater and seawater, soil, sewage, hospital equipment, contact lenses and lens solutions

1. Species that causes Acanthamoeba keratitis

2. Diagnosis of the disease?

3. Treatment of the disease?

4. What medication to avoid?

1. Acanthamoeba spp.

2. Epithelial biopsy, corneal scrapings, culture, PCR

3. Clotrimazole combined with pentamidine, isethionate, neosporin

4. Topical coticosteroids (retards immune repsonse)

1. Species that causes Granulomatous Amebic Encephalitis (GAE)

2. Treatment

1. Acanthamoeba spp.

2. Amphotericin B, Penamidine, Sulfadiazine, Flucytosine, Fluconazole, Itraconazole

1. Species

2. Stage

3. Three stages of life cycle

4. Vegetative forms

5. This species has two forms of trophozoites

6. What are these forms, and which one is found in humans.

7. Environment where they thrive best

8. Entry pathway to the body

1. Naegleria spp.

2. Trophozoites in ameboid

3. Cysts, trophozoites, flagellated forms.

4. Ameba (trophozoite form) and flagellate (swimming form)

5. Naegleria fowleri

6. Ameboid and Ameboflagellate. Ameboid is found in humans.

7. Hot springs and other warm environment (thermophilic)

8. Nasal mucos

1. Also know as the brain-eating amoeba.

2. What disease does it cause?

1. Naegleria fowleri

2. Primary Amebic Meningoencephalitis (PAM)

1. Species

2. Infective stage

3. Diagnostic stage

4. Location in this infective stage

1. Naegleria fowleri

2. Trophozoite

3. Trophozoites in CSF and brain tissue

4. Human tissues and CSF

1. Species that causes Primary Amebic Meningoencephalitis (PAM)

2. Diagnosis

3. Treatment

1. Naegleria fowleri

2. Aspirates from suspected infection and introduced into bacteria-seeded agar culture medium

3. Amphotericin B (Blood-Brain Barrier)