Microscopy RP

Using a Microscope: Step 1

Clip slide onto the stage

Using a Microscope: Step 2

Ensure the lowest powered objective lens is over the slide

Using a Microscope: Step 3

Use the coarse adjustment knob to bring the stage up just below the lens

Using a Microscope: Step 4

Look down the eye piece and gradually move the stage downwards using the coarse adjustment knob

Stop when the image is roughly in focus

Using a Microscope: Step 5

To bring the image into focus adjust the fine adjustment knob until you get a clear image

Using a Microscope: Step 6

To observe the image with a higher magnification, change the objective lens to a higher power and readjust the stage using the coarse and fine adjustment knobs

Microscopy Step 1

Use the lowest objective lens to find the biggest field of view

Microscopy Step 2

Use the coarse focus to move the stage

Microscopy Stage 3

Use the fine focus to get a clear image

Microscopy Stage 4

Eyepiece Lens x Objective Lens = Magnification

Microscopy Stage 5

Use the equation Magnification = Image Size/Actual Size

Electron Microscopes

• Have a higher magnification

• Have a higher resolution

Total Magnification Equation

Total Magnification=Eye Piece Magnification x Objective Lens Magnification

Magnification Equation

Magnification=Image/Actual

Actual Size Equation

Actual Size=Image Size/Magnification

Image Size Equation

Image Size=Actual Size x Magnification

Blurry Image

Use the focus dials to make an image clearer