5.3 Measuring Bacterial Growth
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15 Terms
What is the range of time it takes for a cell to divide?
20 minutes to 3 hours
How do bacteria grow?
Bacteria grow through binary fission
Describe the bacterial growth curve
How can we measure turbidity?
We can measure turbidity through a spectrophotometer
What are the advantages of using a spectrophotometer?
Advantage: Results are obtained right away
What are the disadvantages of using a spectrophotometer?
Disadvantages: Cannot be used for very dilute cultures, dead cells are also counted
What is the Petroff Hausser Chamber?
The Petroff Hausser Chamber observed a very small liquid sample under a microscope
What are the advantages of the Petroff Hausser Chamber?
Advantage: Results are obtained right away
What are the disadvantages of the Petroff Hausser Chamber?
Disadvantages: Cannot be used for very dilute cultures, dead cells are also counted
What does CFU mean?
CFU means colony forming units
What are the advantages of plating?
Advantages: Counts only live cells, more accurate
What are the disadvantages of plating?
Disadvantages: Incubations are required, so obtaining the results are after a few days
Dilutions are also required, as plating a sample will lead to a lawn of growth (TNTC)
How many colonies are considered countable on plating?
Ideally 20 to 200 colonies
What is the process of serial dilutions?
A sample is mixed with a diluent (usually water)
That sample with the diluent is further mixed with more diluent, repeating several times
What is the formula for counting colonies in serial dilutions?
CFU / plating factor x 1 / dilution factor