DEHYDRATION, CLEARING, AND INFILTRATION

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Last updated 5:01 AM on 3/8/26
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9 Terms

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DEHYDRATION
The removal of water from the fixed tissue
After fixation and prior to impregnation
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Most common dehydrating agent in the
laboratory
Alcohol
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Recommended ratio of dehydrating agent to tissue
10 to 1
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Recommended ratio of tissue to dehydrating agent
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ETHANOL
Recommended dehydrating agent for routine dehydration of tissues.
o it’s fast acting
o it mixes with water and many
organic solvents
o it penetrates the issue easily
 clear, colorless, & flammable fluid
 not poisonous and inexpensive
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ISOPROPYL ALCOHOL
dehydrating and clearing agent
 substitute for ethanol
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METHYL ALCOHOL
a toxic dehydrating agent
 utilized for blood and tissue films
 also used as a fixative for smear preparations
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BUTYL ALCOHOL
A slow dehydrating agent which produces less shrinkage and hardening than ethanol for plant and animal micro technique recommended for non urgent works
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CLEARING
Removal of dehydrating agent, or alcohol, from the tissue and is replaced with a substance that will dissolve the wax with which the tissue is to be impregnated.
o For example: melted paraffin wax
o the melted paraffin wax will replace
the alcohol, so the removal of
alcohol will result to making the
tissue transparent or translucent
due to their high index of refraction
Making the tissue translucen