General Microbiology Final Lab Exam Flashcards

Vocabulary flashcards covering the General Microbiology Final Lab Exam topics including staining techniques, sterilization, culture methods, and laboratory equipment.

Potato Dextrose Agar

A medium commonly used for the cultivation of fungi in the laboratory.

Bacterial incubation temperature

The temperature required for bacterial incubation, which is $37\,^{\circ}\text{C}$.

Gram Stain groups

A staining method that divides bacteria into two groups: Positive and negative.

Nutrient agar slant

A medium preparation used to maintain stock culture.

Lactophenol

The specific stain used to observe fungi under the microscope.

Fixation

The process of securing bacteria onto a slide so that they are not washed away during staining.

Physiological characteristics for differentiation

Features such as size, color, and shape used for differentiating bacteria.

Bacillus

A bacterial morphology characterized by a rod shape.

Preparation of $100\,\text{ml}$ media ($30\,\text{g/L}$)

The amount of powder media needed is $\frac{30\,\text{g}}{1000\,\text{ml}} \times 100\,\text{ml} = 3\,\text{g}$.

Aseptic techniques

A laboratory process used to maintain a work area free of bacterial contamination.

Autoclave

The best example of mineralization or sterilization using moist heat.

Gram Stain purpose

A procedure used for differentiating bacteria.

Pure culture

A culture that contains only one type of bacteria.

Streak Plate

A Four Quadrant Method used for the isolation of colonies.

Crowding sample

A condition on the slide that prevents the user from obtaining a good observation.

Filtration

A method used to remove microorganisms from fluids.

Compound microscope

The specific type of light microscope used in the laboratory.

Gram Stain order

The correct sequence of reagents: Crystal violet, Iodine, Ethanol, and Safranin.

Flam (Flame)

The method used in order to sterilize the Inoculating steel loop.