bio lab final - chart

sugar fermentation medium

Phenol red “sugar” broth

sugar fermentation control

Escherichia coli

sugar fermentation appearance

yellow broth → acid: bubble in tube → gas

mixed acid fermentation AKA

methyl red (MR)

mixed acid fermentation medium

MR-VP

mixed acid fermentation enzyme

formic hydrogenylase

mixed acid fermentation control

Escherichia coli

mixed acid fermentation appearance

red when reagent is added

mixed acid fermentation reagent

methyl red

Butanediol fermentation AKA

Voges-Proskauer (VP)

Butanediol fermentation medium

MR-VP

butanediol fermentation control

Enterobacter aerogenes

Butanediol fermentation appearance

red when reagent is added

butanediol fermentation reagent

Barritt’s reagents A&B

catalase production medium

nutrient agar

catalase production enzyme

catalase

catalase production control

Staphylococcus aureus

catalase appearance

bubbles when reagent added

catalse reagent

H2O2 - hydrogen peroxide

oxidase production medium

TSA agar or similar

oxidase enzyme

oxidase

oxidase control

Pseudomonas aeruginosa

oxidase appearance

dark purple spot when reagent added

oxidase reagent

oxidase test reagent

nitrate reduction medium

nitrate broth

nitrate reduction enzyme

nitratase

nitrate reduction control

Escherichia coli

nitrate reduction appearance

red when reagent added

nitrate reduction reagents

nitrate reagents A&B

starch hydrolysis medium

starch agar

starch hydrolysis enzyme

amylase

starch hydrolysis control

Bacillus subtilis

starch hydrolysis appearance

clear zone around growth when reagent added

starch hydrolysis reagent

iodine solution (Gram’s)

casein hydrolysis medium

skim milk agar

casein hydrolysis enzyme

caseinase

casein hydrolysis control

Bacillus subtilis

casein hydrolysis appearance

clear zone around growth

lipid hydrolysis medium

spirit blue agar

lipid hydrolysis enzyme

lipase

lipid hydrolysis control

Staphylococcus aureus

lipid hydrolysis appearance

blue and clearing of fat droplets around growth

tryptophan hydrolysis AKA

indole test

tryptophan hydrolysis medium

tryptose broth (or SIM medium)

tryptophan hydrolysis enzyme

tryptophanase

tryptophan hydrolysis control

Escherichia coli

tryptophan hydrolysis appearance

red layer floating on top when reagent is added

tryptophan hydrolysis reagent

Kovac’s reagent

urea hydrolysis medium

urea broth

urea hydrolysis enzyme

urease

urea hydrolysis control

Proteus vulgaris

urea hydrolysis appearance

fuschia, hot pink

H2S production medium

SIM medium

H2S production enzyme

cysteine desulfurase

H2S control

Proteus vulgaris

H2S appearance

black precipitate

citrate utilization medium

Simmon’s citrate agar slant

citrate utilization enzyme

citratase

citrate utilization control

Enterobacter aerogenes

citrate utilization appearance

growth and blue

phenylalanine deamination medium

phenylalanine agar slant

phenylalanine deamination enzyme

phenylalanase

phenylalanine deamination control

Proteus vulgaris

phenylalanine deamination appearance

green slant when reagent added

phenylalanine deamination reagent

10% ferric chloride

IMViC

I= indole, M=methyl red, V(i)=Voges-Proskauer, C=citrate utilization