Microbiology Lecture Review Flashcards

Flashcards to review key vocabulary and concepts from microbiology lectures, focusing on microbial control methods and viable counts.

Aqueous Solution

A chemical that works better in an aqueous solution.

Potency Testing Methods

The number of common methods used to test the potency of a disinfectant.

Autoclave Heat Type

Autoclaves use this type of heat.

Radiation Type

Type of radiation capable of penetrating paper and plastic.

Dessication

Another term for dessication.

Dessication

The type of dimers caused by non-ionising radiation.

Heavy Metal Syphilis Treatment

Heavy metal historically used to treat syphilis.

Handwashing

Method of control exemplified by handwashing.

Thermal Death Point

Lowest temperature at which all microbes are killed in a 10-min exposure.

HEPA Filter P

What does the P in HEPA filter stand for?

Cleanliness Factors

Factors which influence cleanliness.

Pasteurisation

Types of pasteurisation.

Dessication Agents

High concentrations of salt and what else is used for dessication.

Carbolic Acid

Another name for carbolic acid.

Iodophor

Type of compound where iodine is complexed with an organic molecule.

Hyperbaric Chamber Gas

Hyperbaric chambers use which gas in pressurised form?

Sterilants

Term used to describe chemicals used for sterilisation.

Sterilization

Term used to denote complete removal or killing.

Surfactants

What do surfactants do to the surface tension of water?

Slow-Release Chlorine Compounds

Types of compounds which facilitate slow release of chlorine.

Argyria Metal

Which heavy metal causes Argyria?

Autoclave Air Replacement

In an autoclave, air is replaced by this.

Pasteurization Organisms

Type of organisms reduced by pasteurisation.

Beta-Propionolactone Metabolite

Beta-propionolactone is metabolised quickly to this acid.

Viable Count

Measurement of living, reproducing population of bacteria.

Viable Count Colony Range

Count colonies on plates with 30-300 colonies for accurate viable counts.

Dilution in Viable Counts

To obtain the appropriate colony number for viable counts, the sample should always be diluted.

Pour Plate Method

A viable count method where the sample is mixed with molten agar and poured into a sterile plate.

Spread Plate Method

A viable count method where the sample is spread evenly over the surface of an agar plate.

CFU/mL Calculation

CFU/mL = (Number of Colonies) / (Volume Plated * Dilution Factor)