Restriction Mapping of Plasmid DNA

These flashcards cover essential vocabulary and concepts related to the restriction mapping of plasmid DNA, methods involved, and tools used in the lab experiment.

Restriction Enzymes

Enzymes produced by bacteria that recognize and cut specific sequences of DNA.

Sticky Ends

DNA ends that have a 4 base pair overhang created after cuts by specific restriction enzymes, such as EcoRI.

Blunt Ends

DNA ends that do not have overhangs, created by restriction enzymes like Hae III.

Restriction Map

A map showing the relative positions of restriction sites within a DNA molecule based on fragment sizes produced after digestion.

Plasmids

Small circular DNA molecules commonly used as vectors for gene cloning.

Gel Electrophoresis

A method for separating DNA, RNA, or proteins based on size by applying an electric current to a gel.

Molecular Weight Ladder (MW Ladder)

Contains DNA fragments of known sizes used to determine the sizes of unknown DNA fragments in gel electrophoresis.

CutSmart Reaction Buffer

A buffer solution used in restriction enzyme reactions that contains potassium acetate, Tris-acetate, magnesium acetate, and BSA.

Agarose Gel Preparation

The process of making a gel used for electrophoresis, typically containing agarose and a buffer solution like TAE.

Standard Curve

A plot used to relate the distance fragments migrate in gel electrophoresis to their size (molecular weight).