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What is plasmolysis?
Where the protoplasm of the plant cell begins to shrink away from the cell wall.
What is incipient plasmolysis?
Where the water potential of the cytoplasm is the same as the solution.
The point at which plasmolysis just begins to occur.
When 50% of cells in a tissue are plasmolysed.
Why are the plant cells left in the mineral salt solution for 20 minutes before observation?
To allow time for osmosis to reach equilibrium.
What is the first step in the procedure of this practical.
Transfer a small set volume of each mineral salt solution into a watch glass. Place one of the sections of plant tissue into the watch glass and leave for 20 minutes.
What is the second step in the procedure of this practical.
Remove each section using forceps. Mount in a drop of the corresponding solution on a microscope slide and cover with a coverslip.
What is the third step in the procedure of this practical.
Observe 25 cells and make note of how many are plasmolysed.