Agarose Gel Electrophoresis

Flashcards covering key concepts related to Agarose Gel Electrophoresis.

Agarose Gel Electrophoresis

A technique used to separate DNA based on size.

DNA Charge

DNA has a negative charge due to its phosphate backbone.

Agarose Gel Composition

Agarose gel is made from agarose powder mixed with a buffer solution.

Gel Visualization Dye

Gel green dye binds to DNA and fluoresces under UV light for visualization.

Loading Buffer Components

6X Loading Buffer contains Bromophenol Blue for color and Glycerol for weight.

DNA Ladder

A DNA marker used to estimate the size of DNA fragments on a gel.

Electrophoresis Direction

DNA migrates from the cathode (black) to the anode (red) in an electrophoresis chamber.

Polymerization of Agarose

Agarose cools to form a flexible gel, allowing for DNA separation.

Electrophoresis Buffer Purpose

Buffer is used instead of water to maintain pH and conductivity during electrophoresis.

Small DNA Fragments

Smaller DNA fragments move faster through the gel compared to larger ones.