In-class activity: ER Protein Transport and Sorting
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5 Terms
A secreted protein normally contains an N-terminal ER signal sequence. A mutation removes this signal sequence. Where will the protein most likely be found after translation?
Cytosol
3 multiple choice options
A researcher inhibits the signal recognition particle (SRP). What is the most immediate effect on proteins destined for secretion?
They fail to enter the ER and remain in the cytosol
3 multiple choice options
A single-pass transmembrane protein has an N-terminal ER signal sequence and a stop-transfer sequence. The stop-transfer sequence is deleted. What happens to the protein?
It is fully translocated into the ER lumen
3 multiple choice options
A protein contains an internal ER signal sequence (not at the N-terminus). What is the most likely outcome?
The protein becomes a transmembrane protein
3 multiple choice options
A protein contains both an ER signal sequence and a nuclear localization signal (NLS). Where will the protein ultimately localize?
ER (secretory pathway)
3 multiple choice options