biology biological diversity

modules 4 and 6

plant passive defences physical

waxy cuticle, cellulose cell wall, stomata, bark, casparian strip

passive plant defences chemical

e.g catechol, bark resin, enzyme inhibitors like tannins, yeast on leaf surface

active plant defences

hypersensitivity- rapid death of tissue surrounding infection

reinforced cell walls (callose and lignin deposition)

plasmodesmata narrowing by callose

cytoplasm of nearby cells grows into xylem to make a callose wall

sieve pores of phloem blocked up by callose so sap can’t be transported

conservation agreements

CITES CBD CSS

what does CITES stand for

the convention on international trade in endangered species

what did CITES entail

-regulates trade in wild animal and plant species

-illegal to sell endangered species. limits trade made from endangered animals

-raise awareness

what does CBD stand for conservation agreement

the Rio convention on biological diversity

what did the Rio convention entail

-How to use resources sustainably

-how concerning biodiversity is a global responsibility

-government guidance

what does CSS stand for conservation

the countryside stewardship scheme

what does the countryside stewardship scheme do

-improve and extend wildlife habitats through management

- pay farmers to help regenerate hedgerows and wildflower borders, grassy strips

-use their flock to graze very fast growing species

in situ conservation

protecting species in their natural habitat for example marine conservation zones and wildlife reserves

ex situ conservation

protecting species outside of their natural habitat for example seed banks botanic gardens and zoos

signalling molecules in plant defence

phytoalexins, salicylic acid, ethylene, pheromones

what do phytoalexins do in plant signalling

disrupt pathogen metabolism, delay pathogen reproduction, disrupt bacterial cell surface membranes, stimulate the release of chitinase

role of salicylic acid in plant signalling

migrates through plant to unaffected areas and activates defence mechanisms that protect the plant against pathogens for a period. long-term protection called systemic acquired resistance

what does ethylene do in plant signalling

plants secrete it when pathogens are attacking. it vaporises and stimulates other leaves on the plant to react and other plants

primary non specific animal defences

skin/sebum, expulsive reflexes, mucous membranes, lysozymes, stomach acid

lysozymes role and where found

break down bacterial cell walls. found in blood, sweat, tears, breast milk

what is commensal bacteria and where is it found

non-pathogenic bacteria found in and on humans. E. coli and Candida albicans are common. found in skin, mouth, and intestines.

why is commensal bacteria good

compete with pathogenic microorganisms for nutrients and space and prevent them from invading host tissue

second line of defence molecules

phagocytes and antimicrobial proteins

second line of defence responses animal

blood clotting, inflammation, wound repair, phagocytosis

blood clotting cascade overview

platelets trigger a reaction cascade that results in the formation of fibrin which forms a scab

the inflammatory response charecteristics

redness, swelling, heat, pain, loss of function

what is inflammation

a local repsonse to infection and tissue damage

what happens in the inflammatory response

mast cells secrete the cell signalling molecule histamine, which causes vasodilation (increases blood flow through capilliaries) and fluid enters the tissues, creating swelling. some plasma leaves the blood and phagocytes, and cells release cytokines that trigger an immune response in the infected area

what are cytokines

cell signalling proteins that stimulate inflammation and an immune response

what are interleukins

a type of cytokine. IL-1 and IL-6 promote inflammation, IL-1 targetting the brain causing drowsiness and fever.

process of wound repair

scab is formed from blood clotting, stem cells divide by mitosis underneath. blood vessels form, collagen is produced, granulation tissue forms to fill the wound, stem cells move over the new tissue and produce epithelial cells, contractile cells cause wound contraction, unwanted cells die

Blood clotting

-Collagen fibres are exposed

-platelets recognise and stick to them

-platelets release prothrombin activator

-prothrombin becomes thrombin

-thrombin cleaves fibrinogen into fibrin.

-fibrin fibres reinforce clot

what is a phagocyte

white blood cells produced in bone marrow that perform phagocytosis

phagocyte function

remove dead cells and pathogens. non-specific immune response

types of phagocyte

neutrophil, macrophage, dendritic cells

what is phagocytosis

the process of recognising and engulfing cells or particles

neutrophil definition

short lived phagocytes that act in the body tissues. multilobed nucleus. released in large numbers during infection.

neutrophil mode of action

-attracted to pathogens or cells under attack by chemotaxis.

-recognise antibody molecules on pathogens and attaches to them.

-cell surface membrane extends out and around the pathogen, engulfing it and trapping the pathogen in a phagocytic vacuole.

-endocytosis. lysosome fuses to form phagolysosome, killing and digesting the pathogens, dying themselves.

what is a sign of dead neutrophils

pus

what is a macrophage

• large long-lived phagocytes.

• can become APCs

where are macrophages produced

bone marrow

where are neutrophils produced

bone marrow

what do macrophages mature from

monocytes, developing into macrophages when they leave the blood

where do macrophages reside

they reside in lungs, liver, spleen, kidney, lymph nodes

macrophages mode of action

initiate the specific immune response. don’t completely destroy pathogens, but take the antigens and become antigen presenting cells, whihc can be recognised by lymphocytes

what are dendritic cells

large phagocytic cells with lengthy extensions. large surface area.

where are dendritic cells found

throughout the body

what is the role of antigen presenting cells

t cell activation to provide a specific immune response

what can an antigen presenting cell present

the antigens from toxins, foreign cells and ingested pathogens

what are opsonins

chemicals that bind to and tag foreign cells, making them easily recognisable to phagocytes

what is a phagosome

a vesicle that forms within a phagocyte

what is a lysosome

vesicles that contain enzymes capable of breaking down biological polymers

what cells are involved in the cellular response

T cells (helper, killer/cytotoxic, regulatory, memory)

macrophage

where do t cell mature

the thymus gland

where to b cells mature

the bone marrow

where do t lymphocytes orginate from

they originate in the bone marrow

what happens when t cells mature

gain specific cell surface receptors called t cell receptors, which have a similar structure to antibodies

t cell role in immune response

-complementary t cells binds to antigen presenting cell, and divide by mitosis to produce clones

-differentiate into t helper and t killers

-clonal selection of b cells

-release interleukins that trigger phagocytosis and b cells

what is a t helper cell

a t cell that release interleukins that increase phagocyte activity and activate b cells

what is a t killer cell

search for antigen presenting cells and attatch to the antigens and secrete toxic substances to kill infected cells and the pathogen inside. secrete perforins

what is a perforin

a glycoprotein responsible for pore formation in cell membranes

what is a t memory cell

a t cell that remains in the blood after infection ready to be activated for clonal selection if there is reinfection

what is clonal selection

specific b or t lymphocytes containin receptors complementary to a particular antigen are selected and activated

what is clonal expansion

the process of rapid cell division resulting in the multiplication of genetically identical cell clones from a single parent cell

where are b lymphocytes found

the whole body, concentrated in the lymph nodes and spleen

what produces the humoral response

b lymphocytes

b cell activation

b cells with complementary antibody receptors bind to antigen presenting cells. clonal selection. also binds with interleukins and t helper cells

clonal expansion in b cells

activated b cells divide by mitosis to produce clones. some differentiate to plasma cells and some memory cells

plasma cell

a type of b cell that mass produces antibodies. short lived

primary immune response

response to a newly encountered antigen. time delay, weaker, and more short lived

secondary immune response

response to a previously encountered antigen. stronger more long lasting

what happens in the primary immune response

clonal selection and expansion of specific t and b cells, synthesis of antibodies

how long does the primary immmune response take to produce antibodies

10-17 days

why is the secondary immune response quicker

more memory cells present to be selected than the original clone than before so more antibodies can be quickly produced

what do b memory cells divide into

plasma cells and more memory cells

types of t memory cells

memory helper t cells, memory killer t cells

what are antibodies

globular glycoproproteins called immunoglobulins

what structure do antibodies have

quaternary. y shape. two heavy polypeptide chains disulfide bonded to two light polypeptide chains. constant and variable region

how many types of mammalian antibody are there

there are 5 types

what is the constant region of an antibody for

binding to b cells

what is the variable region of an antibody for

binding to specific antigens to form antigen-antibody complex

what is the hinge region of an antibody

gives flexibility to the antibody and allows antigen binding sites at different angles

antibodies function

destroy pathogens directly or by activating other immune cells. anti-toxins, opsonins, agglutinins

antibodies as antitoxins

binding to toxins produced by pathogens neutralising them

antibodies as opsonins

attatch to pathogens, making them easily identifiable to phagocytes. called opsonisation

antibodies as agglutinins

cause pathogens with antigen antibody complexes to clump together. called agglutination. makes phagocytosis easier

antibodies deactivating pathogens

attatch to bacteria flagella making them less active. phagocytosis easier

antibodies stimulating cell lysis

work with complement protein to cause holes in cell wall and lysis

active natural immunity and example

immune system naturally exposed to pathogen and produces antibodies against it. eg catching a cold

active artificial immunity example

vaccination is an example

active immunity

antigen enters the body triggering a specific immune response

passive immunity

antibodies not produced by infected person

passive natural immmunity example

antibodies passed from mother to child through breastmilk

passive artificial immunity example

injection of antibodies or tetanus antitoxin

what is an autoimmune disease

the immune system attacks one or more self-antigens. targeted towards a single organ or the entire body

rheumatoid arthritis

autoimmune disease that solely affects the joints. begins in the fingers and hands, spreading to shoulders and elsewhere.

rheumatoid arthritis symptoms

muscle spasms, inflamed tendons, lethargy, constant joint pain

causes of autoimmune disease

not fully understood. inheritable

herd immunity

indirect protection from an infectious disease when a population is immune through vaccination or previous infection

ring vaccination

when a person gets an infection, those in close contact are vaccinated against it

what could a vaccine contain

dead or inactivated pathogens, attenuated pathogen strains, harmless version of toxin, isolated antigens, genetically engineered antigens

what is an attenuated pathogen

a weakened version