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Complex I
NADH Q Oxidoreductase: NADH/NAD+ —→ FMN/FMNH2 —→ 4 Fe/S clusters —→ Q/QH2 (ubiquinone to ubiquinol)
How many H+ pumped in Complex I?
4 from matrix into IMS
Complex II
Succinate Q Reductase: Succinate/Fumarate (oxidation) —→ FAD/FADH2 —→ 2 Fe/S clusters —→ Q/QH2
How many H+ pumped in Complex II?
0
Complex III
QH2 Cytochrome c Oxidoreductase: QH2 —→ Fe/S cluster —→ heme c1 —→ heme c (Cytochrome c) OR QH2 —→ heme b —→ heme c (Cytochrome c)
How mant H+ pumped in Complex III
4; 2 from matrix to IMS and 2 from QH2 to IMS
Complex IV
Cytochrome c Oxidase: (2) Cytochrome c —→ Copper unit a —→ heme a —→ heme a3 —→ Copper unit b —→ O2 OR (2) Cytochrome c —→ (2) H20
How many H+ pumped in Complex IV?
2 from matrix into IMS (if you use 2 Cyt c)
Exam Style Q
2 ATP from glycolysis and 2 ATP from Krebs
Malate/aspartate shuttle (h, k, and l) = 2 NADH x 2.5 ATP V.S. Glycerol 3 phosphate shuttle (m and b) = 2 NADH x 1.5 ATP
Glycolysis = 2 NADH x 2.5 ATP
PDC = 2 NADH x 2.5 ATP
Krebs = 6 NADH x 2.5 ATP and 2 FADH2 × 1.5 ATP
Total = 32 or 30 depending on Step 2