Biotech II Lecture 20 Different Types of Stem Cells
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8 Terms
how do you isolate es cells
pierce outside of a blastocyst with a pipet and extract a single blastomere
how do you create a stem cell line from a single blastomere
put it in appropriate culture conditions
embryonic stem (es) cells vs adult stem cells
es cells are pluripotent and adult stem cells are multi- or unipotent
characteristics of true adult stem cells (tissue stem cells)
long-term self-renewal capabilities, maintains specific tissue for life, generates multiple cell types in that tissue
ex. hematopoietic stem cells
characteristics of multipotent stromal cells (mesenchymal stem cells - MSCs)
connective tissue-associated cells, multipotent differentiation in vitro (in vivo is still being debated), function through signaling and tissue support
characteristics of progenitor cells
more differentiation than true stem cells, limited self-renewal, specific lineage,
ex. EPCs
where are hematopoietic stem cells found and what kind of stem cell are they
hscs are found in the bone marrow and are multipotent adult stem cells