Lab exam II

What type of mutation causes sickle cell anemia?

missense

Which band has the one band

homogeneous -/- or ++

how are (fish) proteins separated

according or molecular weight

name three uses of PCR

forensic science, who’s the daddy, and genetic disease

DNA fingerprinting relies on what variation/DNA sequence?

STR Region

Two individuals with identical DNA finger bonding patterns

twins/ very related

which proteins were affected/limited with sickle cell disease

red blood cells

what does the GA (Glutamic Acid) promote?

amylase

Which irons are required for amylase activity?

Calcium

ABA functions primarily do what?

inhibit seed germination

what does vmax represent

maximum reaction rate

how many dna doubles after 5 PCR cycles

2^5 = 32

purpose of IKI (iodine)

binds to starch to produce a colored complex (change to blue) ; absorbance decreases, starch increases

SDS (Sodium Dodecyl Sulfate) Function

dissolves the lipids (fats) in the cell membranes and the nuclear envelope. It also denatures proteins, "unfolding" them so they no longer function. Think of it like dish soap cutting through grease to get to what’s inside.

chellex function

protects the DNA from being destroyed by enzymes. acts like a chemical magnet that grabs metal ions (specifically Mg 2+). prevents these enzymes from chopping up your DNA sample before you can use it.

what does the gel do

separates proteins in mixers based on molecular weight