ASCP Review

Which Pseudomonas species will have YELLOW pigmentation on blood agar?

A: P.fulva

B: P.fluorescens

C: p.aeruginosa

D: p.mosselii

A: P.fulva

Pseudomonas fulva will show yellow pigmentation on blood agar.

Pseudomonas fluorescens has no distinctive appearance on blood agar.

Pseudomonas aeruginosa will have bluish green, red, or brown pigmentation observed on blood agar.

Pseudomonas mosselii has no distinctive appearance on blood agar.

(reference: LabCE)

According to WHO, diagnosis of acute leukemia is defined by:

A: less than 20% blasts in blood or bone marrow

B: more than 20% blasts in blood or bone marrow

B: more than 20% blasts in blood or bone marrow

Acute leukemias—defined as 20% or more blasts in blood or bone marrow under the World Health Organization (WHO) criteria—are classified according to the predominant neoplastic cell line and thus may be designated as lymphoblastic or myeloid.

(reference: https://www.sciencedirect.com/topics/medicine-and-dentistry/acute-leukemia#:~:text=Acute%20leukemias%E2%80%94defined%20as%2020,designated%20as%20lymphoblastic%20or%20myeloid.)

What tests are part of a BMP?

A: ALB,ALP,AST,ALT, DBIL, TBIL,TP

B: CHOL,HDL, TRIG, LDL

C: BUN, CREAT, CA, CO2, GLU, LYTES

D: BUN, CHOL,ALB, CO2, DBIL

C: BUN, CREAT, CA, CO2, GLU, LYTES

The basic metabolic panel (BMP) is a blood test that gives doctors information about the body's fluid balance, levels of electrolytes like sodium and potassium, and how well the kidneys are working.

reference: clincial rotation

What tests are part of a Lipid panel

A: ALB,ALP,AST,ALT, DBIL, TBIL,TP

B: CHOL,HDL, TRIG, LDL

C: BUN, CREAT, CA, CO2, GLU, LYTES

D: BUN, CHOL,ALB, CO2, DBIL

B; CHOL,HDL, TRIG, LDL

A lipid panel is a blood test that measures lipids—fats and fatty substances used as a source of energy by your body.

reference: clinical rotation

The TIBC (total iron binding capacity) is an indirect measure of what iron transport molecule?

A: ferritin

B: transferrin

C: heme

D: hemoglobin

B: transferrin

The measurement of the maximum concentration of iron that it can bind is called the total iron-binding capacity (TIBC). The TIBC test serves as a good indirect measurement of transferrin.

(reference: hematology course)

Which of the following is a microcytic anemia?

A: folate deficiency

B: iron deficiency

C: aplastic anemia

D: B12 deficiency

B: Iron deficiency anemia

[TICS]:Thalassemia, IDA, Chronic disease, Sideroblastic anemia (lead poisoning)

(reference: hematology course)

which of the following enterobacteriaceae produce shiga-like toxin?

A: k.pneumoniae

B: e.coli

C: salmonella typhimurium

D: e.clocae

B: e.coli

strains of e.coli produce shiga-like toxins (SLT I and SLT II)

think about e.coli O157:H7

reference: CLS review book

Sheep blood agar is the primary isolation medium for all of the following except:

A: staph. aureus

B: beta strep

C: haemophilus

D: pseudomonas

C: haemophilus

haemophilus primary isolation medium is chocolate agar

reference: CLS review book

The MSDS for hazardous chemicals address which of the following conditions:

A: physical characteristics

B: storage

C: health hazards

D: all of the above

D: All of the above

MSDS documents should describe the chemical and physical characteristics, safe handling, storage, and potential health hazards of reagents used in the laboratory.

reference: CLS review book

India ink stain is used for which organism

A: aspergillus niger

B: cryptococcus neoformans

C: histoplamsa capsulatum

D: candida albicans

B: cryptococcus neoformans

Positive india ink stain shows yeast cells with a surrounding clear area. see image

reference: CLS review book

(chemistry) While verifying results, you come across extremely high Na and Cl values. They do not match previous results and all the other tests are done are within range. What are your next steps?

A: Verify results as is

B: Cancel test and send for redraw

C: Call the nurse for suspicious saline contamination

D: Verify results but insert contamination comment

C: Call the nurse for suspicious saline contamination

You should be suspicious of saline contamination in this case. The best practice is to call a nurse for more information and then submit it for redraw. Don't automatically assume contamination and verify with comment without confirmation.

reference: clinical rotation

Which of these donors are eligible to donate today?

A: 14year old healthy male

B: 18year old female with the flu

C: 25year old female 2 weeks postpartum

D: 20year old male with asthma

D: 20year old male with asthma

Age: at least 17 (or 16 with parent consent)

Cold/flu: cannot donate

Pregnant: cannot donate- only 6 weeks after birth

Asthma: as long as you don't have any limitations, you can donate.

reference: red cross

Which component has the longest expiration date:

A: frozen rbcs

B: cryoprecipitate

C: FFP

D: platelet concentrate

A: Frozen rbcs

frozen rbcs = 10 years

cryoprecipitate = 1 year

FFP = 1 year

platelet concentrate = 5 days

reference: CLS review book

Trauma patient is having a severe hemolytic reaction minutes after receiving a blood transfusion. Which is the most probable cause?

A: volume overload

B: iron overload

C: ABO incompatibility

D: Anti-jka incompatibility

C: ABO incompatibility

An immediate reaction minutes after transfusion is most likely due to an error in ABO. Anti-jka would usually be a delayed reaction.

reference: CLS review book

All of the following are performed on cord blood routinely except:

A: forward typing abo

B: rh type

C: DAT

D: Antibody screen

D: Antibody screen

antibody screens are not performed until 6 months. Babies do not make antibodies until 6 months, any antibodies detected now would be from the mother.

reference CLS review book

Which specimen is the sample of choice for lead testing?

A: whole blood

B: hair

C: serum

D: urine

A: whole blood

although it can accumulate in all choices, best choice is whole blood it gives the greatest sensitivity over time. Lead is rapidly eliminated from plasma/serum.

lavender or tan top preferred.

reference: clinical rotation

Which type of cast signals the presence of chronic renal failure?

A: blood casts

B: fine granular casts

C: waxy casts

D: fatty casts

C: waxy casts

waxy casts form from the degradation of cellular casts. because the casts must remain lodged in the tubule long enough for the granular protein matrix to waxify.

reference: CLS review book

Crystals are seen in gout and pseudogout:

A; calcium pyrophosphate & uric acid

B: uric acid & calcium pyrophosphate

C: oxalic acid & calcium oxlate

D: calcium oxlate & oxalic acid

B: uric acid & calcium pyrophosphate

see image

reference: CLS review book

Which tube type prevents blood from clotting by binding calcium which in turn inhibits the coagulation cascade?

A: EDTA

B: Gel

C: Sodium Heparin

D: Sodium Lithium

A: EDTA

EDTA prevents blood from clotting by binding calcium which in turn inhibits the coagulation cascade. EDTA is found in the lavender, pink, white, royal blue, and tan top tubes.

reference: labce

Which globin chains represent fetal hemoglobin Hgb F?

A: Two alpha, two beta

B: Two alpha, two gamma

C: Four beta chains

D: Four alpha chains

B: Two alpha, two gamma

Two alpha, two beta is normal adult

Hgb A

Two alpha, two gamma is fetal hemoglobin Hgb F

Four beta chains Hemoglobin H

reference labce

Acid phosphatase test helps in diagnosing...

A: acute pancreatitis

B: prostate cancer

C: gout

D: liver failure

B: Prostate Cancer

Acid phosphatase test helps in diagnosing prostate cancer. It should be collected in a gel-separator tube. Serum/plasma should be frozen immediately and transported frozen.

reference labce

The type of hypersensitivity reaction is immediate?

A: Type 1

B: Type 2

C: Type 3

D: Type4

A: Type I Immediate hypersensitivity

Type II Antibody mediated hypersensitivity

Type III Immune complex mediated hypersensitivity

Type IV Cell mediated hypersensitivity

reference labce

Which bacteria is usually associated with Swimmer's ear?

A: Haemophilus aegyptius

B: Pseudomonas aeruginosa

C: Aspergillus niger

D: Burkholderia pseudomallei

B: Pseudomonas aeruginosa

Pseudomonas aeruginosa is the most common bacterial cause of "swimmer's ear". This occurs when water is retained in the outer ear canal, giving the perfect habitat for bacteria (or fungi) to grow.

reference lab ce

______ is a serological marker of ovarian cancer.

A: CEA

B: AFP

C: hCG

D: CA-125

D: CA-125

hCG: marker for the detection of gestational trophoblastic disease. CEA is a marker for colorectal cancer. AFP is often elevated in patients with hepatocellular carcinoma and germ cell tumors. CA-125 is a serological marker of ovarian cancer.

reference labce

Which test is considered a more reliable method of monitoring long-term diabetes control?

A: Random plasma glucose

B: Hgb A1c

C: Glucose oxidase

D: Hexokinase

B: Hgb A1c

Hgb A1c is the most commonly detected glycosylated hemoglobin. HbA1c is a more reliable method of monitoring long-term diabetes control than random plasma glucose.

reference: labce

Calculate this patient's MCV:

Hct:36% and rbc: 3.8x10^6

A: 94.7fL

B: 78.4fL

C: 105.5fL

D: 38.6fL

A: 94.7fL

reference: hematology

Which tests help distinguish shigella from e.coli?

A: hydrogen sulfide & citrate

B: lactose & indole

C: MR & urea

D: VP & Gas

B: lactose & indole

E.Coli : positive for lactose, indole, motile

Shigella: no lactose, indole, nonmotile

reference: CLS review book

ALL (acute lymphoblastic leukemia) is most commonly seen in what age group?

A: 1-15years

B: 20-35years

C: 45-60years

D: 65-80years

A: 1-15years

ALL usually affects children and is the most common type of acute leukemia in this age group

reference CLS review book

When an RBC containing iron granules is stained with prussian blue, the cell is called:

A: spherocyte

B: schistocyte

C: basophil

D: siderocyte

D: siderocyte

reference: CLS review book

The most deficiency cited during laboratory inspections are:

A: improper documentation

B: insufficient work space

C: improper reagent storage

D: improper instrument calibration

A: improper documentation

improper document is the first most cited followed by outdated procedure manuals

reference: CLS review book