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BUN Assay
BUN = (A Unknown ÷ A Standard) × 30, used to determine BUN concentration.
Creatinine Assay
Creatinine = (ΔA Unknown ÷ ΔA Standard) × 5, used to determine creatinine concentration.
Urea Conversion
Urea = BUN × 2.14, used to convert BUN to Urea.
BUN Conversion
BUN = Urea ÷ 2.14, used to convert Urea to BUN.
BUN/Creatinine Ratio
BUN ÷ Creatinine, evaluates renal perfusion/function.
Anion Gap (with K)
Na + K − (Cl + HCO3), used for metabolic acidosis evaluation.
Anion Gap (without K)
Na − (Cl + HCO3), the most common AG calculation.
Calculated Osmolality
2(Na) + Glucose/20 + BUN/3, estimates serum osmolality.
Alternative Osmolality
1.86(Na) + Glucose/18 + BUN/2.8 + 9, a more precise estimate of osmolality.
Osmolal Gap
Measured − Calculated Osmolality, detects unmeasured osmoles.
Sodium (Na) Reference Range
135–145 mmol/L, normal range for sodium.
Potassium (K) Reference Range
3.5–5.0 mmol/L, normal range for potassium.
Chloride (Cl) Reference Range
96–106 mmol/L, normal range for chloride.
Bicarbonate (HCO3) Reference Range
22–29 mmol/L, normal range for bicarbonate.
Calcium (Ca) Reference Range
8.5–10.5 mg/dL, normal range for calcium.
Creatinine Female Reference Range
0.6–1.1 mg/dL, reflects GFR in females.
Creatinine Male Reference Range
0.7–1.3 mg/dL, reflects GFR in males.
Creatinine Clearance Female
95 ± 20 mL/min, indicates kidney filtration in females.
Creatinine Clearance Male
105 ± 20 mL/min, indicates kidney filtration in males.
Uric Acid Reference Range
2.6–7.2 mg/dL, related to purine metabolism.
Ammonia Reference Range
19–60 mg/dL, related to liver and nitrogen metabolism.