micr 33 lab charts

Gelatin Hydrolysis

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) nutrient gelatin deep tubes

b) gelatin

c) gelatinase

d) N/A

e) amino acids

Starch Hydrolysis

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) starch agar plate

b) starch

c) amylase

d) iodine

e) glucose

Casein Hydrolysis

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) milk agar plate

b) casein

c) caseinase

d) N/A

e) amino acids

Phenol Red Broth Sugar Fermenation

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) phenol red fermentation broth

b) glucose, lactose, sucrose broths

c) fermentation set of enzymes

d) phenol red

e) acids, alcohols, gases (CO2, H2)

Litmus Milk

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) litmus milk

b) casein (protein) & lactose (sugar)

c) caseinase & lactase

d) litmus blue

e) acids, alcohols, ammonia (NH3), soft/hard curd formation

Tryptophan Hydrolysis/Indole

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) 1% tryptone broth

b) tryptophan

c) tryptophanase

d) Kovac’s reagent

e) indole

Methyl Red Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) MRVP broth

b) glucose

c) incomplete fermentation set of enzymes

d) red (+), yellow (-)

e) acetic, lactic, formic acids

Voges-Proskauer Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) MRVP broth

b) glucose

c) incomplete fermentation set of enzymes

d) VP reagent I, VP reagent II

e) acetoin

Citrate Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) Simmon’s citrate agar slant

b) citrate

c) citrase

d) brom thymol blue

e) sodium bicarbonate

Nitrate Reduction Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) nitrate broth w/ Durham tube

b) nitrate (NO3)

c) nitratase (nitrate reductase)

d) nitrate reagent A & B

e) nitrite (NO2), ammonia (NH3), nitrogen gas (N2)

Hydrogen Sulfide Production Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) triple sugar iron (TSI) slant

b) cysteine

c) cysteine desulfurase

d) ferrous sulfate

e) hydrogen sulfide gas (H2S), ferric sulfide (black precipitate)

Urea Hydrolysis Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) urea broth

b) urea

c) urease

d) phenol red

e) ammonia (NH3), CO2

Oxidase Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) tryptose agar or nutrient agar slant

b) oxidase reagent

c) oxidase

d) oxidase reagent

e) indophenol

Catalase Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) tryptose agar or nutrient agar slant

b) 3% hydrogen peroxide (H2O2)

c) catalase

d) 3% hydrogen peroxide (H2O2)

e) oxygen (O2) bubbles & water

Blood Agar Plate

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) blood agar plate (BAP)

b) blood cells

c) hemolysins

d) blood cells

e) hemolysis

m-Staphylococcus Broth Test/Gram Positive Cocci Experimentation Day # 1

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) mannitol salt broth

b) mannitol & salt

c) N/A

d) phenol red

e) acids

Trypticase Soy Broth Test/Gram Positive Cocci Experimentation Day # 2

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) trypticase soy broth

b) soy

c) trypticase

d) N/A

e) amino acids

Mannitol Salt Plate/Gram Positive Cocci Experimentation Day # 2

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) mannitol salt agar

b) mannitol & salt

c) mannitol oxidase

d) phenol red

e) acids

Coagulase Test/Gram Positive Cocci Experimentation Day # 3

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) rabbit plasma

b) plasma

c) coagulase

d) N/A

e) clot

Optochin Sensitivity Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) blood agar plate (BAP)

b) N/A

c) optochin

d) N/A

e) zone of inhibition

Bacitracin Sensitivity Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) blood agar plate (BAP)

b) N/A

c) bacitracin

d) N/A

e) zone of inhibition

CAMP Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) blood agar plate (BAP)

b) blood cells

c) CAMP factor

d) increased hemolysis

e) zone of beta hemolysis

Buffered Azide Glycerol Glucose Broth (BAGG) Test

a) Medium?

b) Substrate?

c) Enzyme?

d) Indicator/Reagent?

e) End Product?

a) BAGG broth

b) glucose & glycerol

c) hexase

d) brom thymol purple

e) acids