lab 10 - molecular id of microbes

PCR

Which of the following is the most widely used molecular tool in biology?

BLAST

Which of the following is the most widely used bioinformatic tool in biology?

16s rRNA

Which genetic locus is typically used to identify bacteria and archaea?

COI

ITS

Which two genetic loci is typically used to identify fungi and other eukaryotes?

Break the cells open to expose the DNA

In DNA extraction, the first step is to

Remove lipids and proteins

In DNA extraction, the second step is to

Precipitate the DNA with alcohol

In DNA extraction, the third step is to

Buffer

Which reagent of PCR stabilizes the molecular reactions?

Taq Polymerase

Which reagent of PCR creates covalent bonds to make the new DNA strand?

Primers

Which reagent of PCR hybridizes with the DNA at the location where amplification will occur?

dNTPs

Which reagent of PCR is the building blocks of the new DNA?

Reverse Transcriptase PCR

Which type of PCR is used to quantify how much RNA is being produced?

Conventional PCR

Which type of PCR is used to amplify large amount of a DNA target and then sequenced?

Real-Time PCR

Which type of PCR is used as a diagnostic to distinguish between different microbes?

Annealing

Which phase of PCR allows for the primers to form hydrogen bonds with the single stranded DNA

Denaturation

Which phase of PCR raises the temperature to break the DNA into single strands?

Extension

Which phase of PCR uses the Taq polymerase to add complementary nucleotides to the single strand of DNA

Extension

Which phase of PCR has an optimal temperature at 72 degres Celsius?

Denaturation

Which phase of PCR has an optimal temperature at 95 degres Celsius?

Annealing

Which phase of PCR has an optimal temperature at 50-60 degres Celsius?

Max or Total Score

This BLAST result is used as a comparison between searches.

Percent Query Coverage

This BLAST result illustrates how much of the inputted sequence is used in the alignment.

Expect Value

This BLAST result is the number of alignments that occur by chance.

Percent Similarity

This BLAST result illustrates how well the inputted sequence matches to sequences in the database.

Percent Similarity

When using a database to identify a microbe, which is the prefered result to use?