Sanger Sequencing and BLAST

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Last updated 7:12 PM on 4/29/26
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5 Terms

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What used in Seq?

Template DNA

Foward Primer

Taq poly

dNTPS

ddNTPS

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ddNTP (Dideoxynucleotides)

  • Lack a 3’ hydroxyl group

  • When added → DNA stops growing

called the chain termination method

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what sequencing gives you?

a DNA sequence

A, T, G, C

this is the 16s rRNA gene sequence

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the 16s rRNA gene

found in all bacteria

easily amplified

has:

conserved regions (same)

variable regions (different → identify species)

1500bp

large databases exist = allows for comparison

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Blast

compares our DNA sequence to known bacterial DNA sequences.