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Which organisms were likely the first forms of cellular life on Earth?
a. Eukaryotes
b. Prokaryotes
c. Fungi
d. Plants
Prokaryotes
Microbial mats are best described as:
a. Sedimentary rocks formed by evaporation
b. Multi-layered sheets of prokaryotes
c. Single-species bacterial lawns
d. Thin layers of fungal hyphae
Multi-layered sheets of prokaryotes
Stromatolites are:
a. Fossilized microbial mats that precipitate minerals
b. Hot spring chimneys
c. Modern photosynthetic algae
d. Rocks formed by cooling lava
Fossilized microbial mats that precipitate minerals
Cyanobacteria are living in the Stromatolites. They were crucial in Earth history because they:
a. Fixed nitrogen abiotically
b. Created the first eukaryotes c. Oxygenated the atmosphere via oxygenic photosynthesis
d. Eliminated ultraviolet radiation directly
Oxygenated the atmosphere via oxygenic photosynthesis
Extremophiles are organisms that:
a. Require extreme conditions to grow optimally b. Are exclusively eukaryotic c. Cannot form biofilms d. Only live in moderate environments
Require extreme conditions to grow optimally
Which location is hypersaline and supports halophilic prokaryotes?
a. Lake Superior b. Arctic Ocean surface c. Amazon River d. Shark Bay in Australia
Shark Bay in Australia
Why is culturing many prokaryotes difficult?
a. They lack DNA b. Over 99% have unknown specific growth requirements or are obligate intracellular/syntrophic c. They only grow at room temperature d. They cannot be seen with microscopes
Over 99% have unknown specific growth requirements or are obligate intracellular/syntrophic
The viable-but-non-culturable (VBNC) state refers to bacteria that:
a. Are dead b. Are dormant under stress and can resuscitate c. Are actively dividing in rich media d. Have lost their chromosomes
Are dormant under stress and can resuscitate
A biofilm is:
a. A fossil record of ancient bacteria
b. A planktonic bacterial culture
c. A surface-attached microbial community embedded in extracellular matrix
d. A colony grown on blood agar
A surface-attached microbial community embedded in extracellular matrix
One reason biofilm bacteria are harder to eradicate than free-living cells is that:
a. They lack cell walls
b. Their EPS (extracellular polymeric substances) matrix impedes diffusion of antibiotics/disinfectants
c. They are always Gram-negative
d. They do not respire
Their EPS matrix impedes diffusion of antibiotics/disinfectants
A typical prokaryotic chromosome is:
a. Linear, multiple, in a nucleus
b. Circular, usually single, in the nucleoid
c. Circular, multiple, in mitochondria
d. Linear, multiple, in the nucleolus
Circular, usually single, in the nucleoid
Which structure enhances attachment and protection from dehydration in some bacteria?
a. Flagellum
b. Capsule
c. Ribosome
d. Endoplasmic reticulum
Capsule
Archaeal membrane lipids differ from bacterial lipids by having:
a. Branched carbon chains (isoprenoids) that ethers to glycerol.
b. Long singular carbon chains (fatty acids) that esters to glycerol
c. No hydrophobic region
d. Sterol-rich bilayers similar to animals
Branched carbon chains (isoprenoids) that ethers to glycerol.
Bacterial cell walls are composed primarily of:
a. Cellulose
b. Chitin
c. Peptidoglycan (murein)
d. Pseudopeptidoglycan
Peptidoglycan (murein)
Gram-negative bacteria stain which color after Gram staining?
a. Purple b. Green c. Blue d. Pink/red
Pink/red
Which component is found in Gram-negative bacteria but not in Gram-positive bacteria?
a. Teichoic acids b. Lipopolysaccharide (LPS) c. Thick peptidoglycan d. Capsule
Lipopolysaccharide (LPS)
Which cell wall polymer anchors Gram-positive cell wall to the plasma membrane?
a. Lipopolysaccharide b. Lipoteichoic acid c. Porin d. Pseudopeptidoglycan
Lipoteichoic acid
Horizontal gene transfer by a bacteriophage that carries bacterial DNA is called:
a. Transformation b. Conjugation c. Transduction d. Binary fission
Transduction
Conjugation typically requires which structure?
a. Mating bridge (Sex pilus) b. Flagellar hook c. Capsule d. Endospore coat
Mating bridge (S3x pilus)
Which set lists major macronutrients required by prokaryotes?
a. C, H, O, N, P, S
b. Fe, Mn, Zn, Cu
c. Se, As, Hg
d. Ni, Mo, Co
C, H, O, N, P, S
Phototrophs differ from chemotrophs in that phototrophs obtain energy from:
a. Organic compounds b. Inorganic chemicals c. Sunlight d. Fermentation only
Sunlight
A pathogen is best defined as a microorganism that:
a. Lives on the skin b. Causes disease in its host c. Is always a bacterium d. Cannot be transmitted
b. Causes disease in its host
An epidemic is:
a. Always present at low levels
b. only if the outbreak is Worldwide
c. High numbers in a population at the same time
d. A zoonotic only disease
High numbers in a population at the same time
The Black Death / The Black Pest was most likely caused by:
a. Vibrio cholerae
b. Yersinia pestis via fleas and pneumonic spread
c. MRSA
d. Influenza virus
Yersinia pestis via fleas and pneumonic spread
Which statement about foodborne disease is TRUE?
a. Now linked only to meat
b. Biofilms on produce/equipment contribute to outbreaks
c. Washing produce removes all pathogens
d. Only viruses cause outbreaks
Biofilms on produce/equipment contribute to outbreaks
Why is MRSA of particular concern?
a. It is a virus b. Resistant to many antibiotics; spreads in community and hospitals c. Cannot colonize skin d. Only mild disease
Resistant to many antibiotics; spreads in community and hospitals
A major driver of antibiotic resistance is:
a. Underuse in livestock
b. Low mutation rates
c. Imprudent overuse/misuse selecting resistant forms
d. Refusal of vaccines
Imprudent overuse/misuse selecting resistant forms
Zoonoses are diseases that:
a. Only infect humans b. Originate in animals but can infect humans c. Are caused only by viruses d. Are always endemic
Originate in animals but can infect humans
Biofilm infections are difficult to treat because:
a. Biofilms lack metabolism
b. They are only viral
c. They are always intracellular
d. Matrix and slow growth confer high antibiotic tolerance
Matrix and slow growth confer high antibiotic tolerance
BNF converts atmospheric N2 into:
a. Nitrite b. Nitrate c. Ammonia d. Nitrous oxide
Ammonia
In root nodules, oxygen is buffered by: a. Cytochrome c b. Hemocyanin c. Leghemoglobin d. Myoglobin
Leghemoglobin
Legume–rhizobia symbiosis provides:
a. Water conservation b. Natural nitrogen fertilization c. Inhibition of photosynthesis d. Leaf antibiotics
Natural nitrogen fertilization
Loss of gut microbiota after antibiotics can allow overgrowth of:
a. Clostridium difficile
b. E. coli O157:H7
c. Vibrio cholerae
d. M. tuberculosis
Clostridium difficile
Lactic acid bacteria in yogurt improve safety by:
a. Raising pH b. Lowering pH c. Adding antibiotics d. Removing water
Lowering pH
Using microbes to remove pollutants is: a. Bioremediation b. Pasteurization c. Sterilization d. Biomagnification
Bioremediation
A mercury-detoxifying genus mentioned is:
a. Alcanivorax b. Pseudomonas c. Nitrobacter d. Rhizobium
Pseudomonas
Oil-spill surfactant-producing marine bacterium:
a. Nitrosomonas b. Alcanivorax borkumensis c. Deinococcus radiodurans d. Halobacterium salinarum
Alcanivorax borkumensis
Early biotech foods include:
a. Cheese, bread, wine, beer, yogurt (types of fermentation) b. Steel, plastic, glass c. Antibiotics only d. Silicon chips
Cheese, bread, wine, beer, yogurt
The human microbiome benefits us by all EXCEPT:
a. Pathogen protection b. Digestive/vitamin roles c. Immune training
Causing disease
Which of the following was an advantage for plants moving to land?
a) Higher CO availability ₂ b) More competition for CO₂ c) Less sunlight d) Increased predation
Higher CO availability
What adaptation helps plants prevent desiccation?
a) Thin cuticle b) Lack of roots c) Swimming gametes d) Waxy leaves
Waxy leaves
Why did plants evolve structural support on land?
a) To store water b) To increase photosynthesis c) Because water no longer provided buoyancy d) To avoid predators
Because water no longer provided buoyancy
Which of these is NOT an adaptation to terrestrial life?
a) Thick cuticle b) Swimming sperm c) Seeds d) Pollen
Swimming sperm
In a haplontic life cycle, the dominant stage is:
a) Diploid sporophyte b) Haploid gametophyte c) Both equally dominant d) Triploid hybrid
Haploid gametophyte
Which organism shows a diplontic life cycle?
a) Moss b) Fern c) Homo sapiens d) Green algae
Homo sapiens
Alternation of generations refers to:
a) Switching between sexual and asexual reproduction
b) Alternating between haploid gametes and diploid zygotes
c) Alternating between gametes and spores only
d) Haploid and diploid are both dominant, they are multicellular
Haploid and diploid are both dominant, they are multicellular.
In mosses, the main plant body is:
a) Diploid sporophyte
b) Haploid gametophyte
c) Triploid hybrid
d) Polyploid sporophyte
Haploid gametophyte
In angiosperms, the visible plant is:
a) Sporophyte
b) Gametophyte
c) Zygote
d) Spore
Sporophyte
Which group shows increasing sporophyte independence? a) Mosses b) Ferns c) Algae d) Fungi
Ferns
Why is diploidy advantageous?
a) Reduces genetic variation
b) Masks deleterious alleles
c) Prevents polyploidy
d) Eliminates mutations
Masks deleterious alleles
Which feature is associated with seed plants?
a) Large gametophyte b) Swimming sperm c) Reduced gametophyte d) No alternation of generations
Reduced gametophyte
Which of these is a major driver of complexity in plants?
a) Haploidy b) Gene duplication c) Gametophyte dominance d) Lack of polyploidy
Gene duplication
Polyploidy means:
a) Two sets of chromosomes
b) One set of chromosomes
c) More than two sets of chromosomes
d) No chromosomes
More than two sets of chromosomes
Which is an example of an allopolyploid crop?
a) Wheat b) Arabidopsis c) Moss d) Fern
Wheat
What causes hybrid sterility?
a) Perfect chromosome pairing
b) Chromosome mismatch after fertilization
c) Excess pollen
d) Lack of gametangia
Chromosome mismatch after fertilization
Chromosome doubling restores fertility because:
a) It reduces chromosome number
b) It prevents polyploidy
c) It eliminates gametes
d) It creates homologous pairs for meiosis
It creates homologous pairs for meiosis
Why is polyploidy common in plants?
a) Plants have strict species recognition
b) Plants avoid genome duplication
c) Plants cannot hybridize
d) Plants lack centralized species recognition
Plants lack centralized species recognition
Which crop is octoploid?
a) Wheat
b) Strawberry
c) Cotton
d) Tobacco
Strawberry
Which process can instantly create a new species?
a) Mutation b) Polyploidy c) Self-pollination d) Spore formation
Polyploidy
Perfect flowers have:
a) Only stamens
b) Only carpels
c) Both stamens and carpels
d) No reproductive organs
Both stamens and carpels
Which structure becomes fruit after fertilization?
a) Ovule
b) Ovary
c) Stigma
d) Sepal
Ovary
Which part of the flower produces pollen? a) Carpel b) Stamen c) Ovary d) Style
Stamen
What protects seeds and aids dispersal?
a) Flower b) Leaf c) Fruit d) Stem
Fruit
Which of the following is NOT a general characteristic of animals?
a) Multicellularity b) Heterotrophy c) Active movement d) Presence of rigid cell walls
Presence of rigid cell walls
What structural protein holds animal cells together?
a) Keratin b) Collagen c) Cellulose d) Chitin
Collagen
Which junction prevents leakage of fluids between cells?
a) Gap junction b) Desmosome c) Tight junction d) Adherens junction
Tight junction
Which junction allows direct communication between animal cells?
a) Gap junction b) Tight junction c) Desmosome d) Adherens junction
Gap junction
Which tissue type is unique to animals for movement?
a) Muscle and nervous b) Epithelial and connective c) Vascular and dermal d) Cartilage and bone
Muscle and nervous
Flying is unique to which kingdom?
a) Plants b) Fungi c) Animals d) Protists
Animals
Sponges lack which feature?
a) Cells b) Proteins c) DNA d) Tissues
Tissues
Which of these animals is sessile?
a) Oyster b) Bird c) Fish d) Frog
Oyster
Which of these is NOT a type of cell junction in animals?
a) Gap junction
b) Desmosome
c) Tight junction
d) Plasmodesmata
Plasmodesmata
Which of the following is a heterotroph?
a) Oak tree b) Algae c) Cat d) Cyanobacteria
Cat
Which phylum includes organisms with stinging cells?
a) Porifera (Sponges)
b) Cnidaria (Jellyfish)
c) Platyhelminthes (Flatworms)
d) Rotifera (Wheel animals)
Cnidaria (Jellyfish)
Which phylum includes sponges?
a) Cnidaria b) Porifera c) Annelida d) Mollusca
Porifera
Which phylum includes flatworms?
a) Platyhelminthes
b) Nematoda
c) Rotifera
d) Annelida
Platyhelminthes
Which phylum includes microscopic animals?
a) Rotifera
b) Nematoda
c) Cnidaria
d) Mollusca
Rotifera
Which phylum includes roundworms?
a) Annelida b) Nematoda c) Platyhelminthes d) Arthropoda
Nematoda
Which phylum includes insects?
a) Arthropoda b) Mollusca c) Echinodermata d) Chordata
Arthropoda
Which phylum includes sea stars?
a) Echinodermata b) Cnidaria c) Mollusca d) Chordata
Echinodermata
Which phylum includes Octopus?
a) Mollusca b) Arthropoda c) Cnidaria d) Chordata
Mollusca
Which phylum includes mammals?
a) Mollusca b) Arthropoda c) Echinodermata d) Chordata
Chordata
Which type of symmetry do jellyfish exhibit?
a) Bilateral b) Spiral c) Asymmetrical d) Radial
Radial
Flatworms exhibit which type of symmetry?
a) Radial b) Bilateral c) Asymmetrical d) Non
Bilateral
Which group has no true tissues?
a) Cnidaria b) Porifera c) Platyhelminthes d) Nematoda
Porifera
Which group has an exoskeleton made of chitin?
a) Mollusca b) Arthropoda c) Echinodermata d) Chordata
Arthropoda
Animal eggs are generally:
a) Smaller than sperm b) Same size as sperm c) Larger than sperm d) Motile
Larger than sperm
What is the first stage after fertilization in animals?
a) Gastrula b) Blastula c) Larva d) Zygote
Zygote
Which stage involves inward folding to form a hollow sac? a) Blastula b) Gastrula c) Larva d) Neurula
Gastrula
Which germ layer forms muscles and bones?
a) Ectoderm b) Mesoderm c) Endoderm d) Exoderm
Mesoderm
Which germ layer forms the nervous system?
a) Ectoderm b) Mesoderm c) Endoderm d) Neural crest
Ectoderm
Which germ layer lines internal organs?
a) Ectoderm b) Mesoderm c) Endoderm d) Neural crest
Endoderm
What is the process of forming the neural tube called?
a) Gastrulation b) Neurulation c) Organogenesis d) Cleavage
Neurulation
The common ancestor of animals was probably:
a) A single-celled protist
b) A colonial protist
c) A sponge
d) An alga
A colonial protist
Choanoflagellates are:
a) Plants b) Colonial protists c) Animals d) Fung
Colonial protists
Which kingdom includes animals?
a) Plantae b) Fungi c) Opisthokonta d) Protista
Opisthokonta
Which group is entirely terrestrial?
a) Arthropoda b) Onychophora c) Mollusca d) Chordata
Onychophora
Which three phyla dominate marine and terrestrial life?
a) Porifera, Cnidaria, Platyhelminthes
b) Echinodermata, Mollusca, Arthropoda
c) Nematoda, Rotifera, Annelida
d) Arthropoda, Mollusca, Chordata
Arthropoda, Mollusca, Chordata
Which feature evolved for active movement?
a) Rigid cell walls
b) Muscle and nerve tissue
c) Photosynthesis
d) Sessile lifestyle
Muscle and nerve tissue
Animals are:
a) Photoautotrophs
b) Chemoheterotrophs
c) Chemoautotrophs
d) Photoheterotrophs
Chemoheterotrophs