Microbiology Proficiency 2

Tests included in the IMViC

indole, MR, VP, Citrate

Examples of organisms that are A/A

Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae

Examples of organisms that are K/A

Salmonella typhi, Shigella dysenteriae, Proteus mirabilis

Examples of organisms that are K/K

Pseudomonas aeruginosa, Burkholderia cepacia

What temperature is Yersinia enterocolitica incubated at

25-30C

Criteria for enterics

ferment glucose, oxidase negative, reduce nitrates to nitrites

What are the 2 main organisms that produce H₂S

Salmonella typhi, Proteus mirabilis

What is the color of the butt and slant for A/A for TSI

yellow

What is the color of the butt and slant for K/A for TSI

red, yellow

What is the color of the butt and slant for K/K for TSI

red

Examples of slow lactose fermenters

Serratia marcescens, Yersinia enterocolitica

Example of organism that is Indole positive

Escherichia coli

Example of organism that is MR positive

Escherichia coli

Example of organism that is VP positive

Klebsiella pneumoniae

Example of organism that is Citrate positive

Klebsiella pneumoniae

What metabolite is detected in the Urease test

ammonia

LIA result of Proteus Mirabilis

R/A with H2S

LIA result of Salmonella typhi

K/K with H2S

Appearance of slant and butt of K/K on LIA

purple

Appearance of slant and butt of R/A on LIA

Burgundy, yellow

Appearance of slant and butt of K/A on LIA

purple, yellow

What does Klebsiella pneumoniae look like on agar

snot on plate

What is the appearance of positive Indole, MR, VP

red

What is the appearance of negative Indole, MR, VP

yellow

What metabolite is converted to Indole

tryptophan

What is detected in the Methyl Red test

mixed acid fermentation

What is detected in the VP test

acetoin

What is detected in the Citrate test

if the organism uses Sodium citrate as its sole carbon source, inorganic ammonium salts as its sole nitrogen source

What API test also looks at sugar fermentation

E

How does API NE look for carbohydrate utilization

assimilation

Appearance of lactose fermenter on MAC

pink

Appearance of nonlactose fermenter on MAC

yellow

Examples of lactose fermenters

Escherichia coli, Klebsiella pneumoniae

Examples of nonlactose fermenters

Pseudomonas aeruginosa, Burkholderia cepacia

Screening test to differentiate lactose from nonlactose fermenter

oxidase

Confirmatory test to differentiate lactose from nonlactose fermenter

TSI

What agar is this

Hektoen enteric