RS - Day 2

Which is true regarding sectioning

I. Sections can stay in the water bath even for long period of time

II. Paraffin oven is maintained at a temperature of 2-5C above the melting point of the paraffin wax

II only

If the sections roll up on cutting, they adhere and get broken against the knife edge. What would be the possible reason behind this?

A. Knife is dull

B. Knife is blunt

C. Paraffin wax is too hard

D. Knife is tilted too much

B. Knife is blunt

The impregnating agent does not require dehydration and clearing

Gelatin

Commonly used in cytology sample and the use of spray fixative

Coating fixation

Lower melting point of 46° to 48°C, but it is harder than paraffin wax.

a. dimethyl sulfoxide

b. embeddol

d. ester wax

d. ester wax

This glutaraldehyde solution for large tissues is less than 4mm thick.

A. 2% solution

B. 5% solution

C. 4% solution

D. 2.5 % solution

D. 2.5 % solution

What retrogressive change is it called when there is a failure to form an opening?

A. Aplasia

B. Hypoplasia

C. Agenesia

D. Atresia

D. Atresia

A medical technologist was washing out excess picric acid

a. 50-70% alcohol

b. tap water

c. acetone

d alcoholic iodine

a. 50-70% alcohol

Freezing temperature for the Heart

A. -7 to -10°C

B. -13 to 16°C

C. -16 to -20°C

D. -20 to 25°C

C. -16 to -20°C

The following statements are true regarding frozen sections, except

A. Shorter turnaround time

B. Can cut serial sections

C. Minimal shrinkage of tissues

D. Sections are thicker

B. Can cut serial sections

Dehydrating agent, the volume should be _______ the volume of the tissue to

ensure…

A. Less than 10 times

B. More than 20 times

C. Less than 20 times

D. Less than 5 times

A. Less than 10 times

When the ball of finger leaves no mark on the tissue block, evaporation, embedding are considered to be:

Complete

Formic acid of what conc. is considered to be an all around best decalcifying agent:

A. 5%

B. 10%

C. 15%

D. 20%

B. 10%

A natural oils found in skin of of citrus

Limonene

Germ cell tumor marker that synthesized by syncytiotrophoblasts choriocarcinoma

HCG

Function of Fixation:

I. Dehydration

II. Preservation

III. Optical Differentiation

All of the above

Tissue constituents are demonstrated in sections by direct interaction with a dye or staining solution

Metachromatic staining

It is used to demonstrate the myogenic component of tumors

A. Desmin

B. Nf

C. Actin

D. Vimentin

A. Desmin

What is the angle formed between the cutting edge of the knife

Bevel angle

Chelating agent that sequesters metallic ions...

EDTA

Touch preparations for Wright-Giemsa

Isopropyl alcohol

It is used for small teeth and small pieces of bones:

Von Ebner’s

Acid decalcifying agents are the most widely used agents for routine decalcification of large amounts of bony tissues because

I. They are stable, readily available

II. Relatively inexpensive

III. Fast acting

All of the above

A volatile substance found in citrus peels

Limonene

Which of the following is true

I. Honing is heel-to-toe

II. Stropping is toe-to-heel

I & II

A retrogressive change wherein there is an incomplete or defective development of a tissue or organ

A. Hypoplasia

B. Aplasia

C. Agenesia

D. Atresia

B. Aplasia

For central nervous system tissues and cytological studies, particularly of smooth muscles and skin

Cedarwood oil

_________ may result in poor cutting of hard tissues and damage to the knife edge during sectioning

Insufficient decalcification

Simplest and least invasive test to obtain specific types of tissue samples?

A. Core needle biopsy

B. Currettings

C. Fine needle aspiration

D. Punch biopsy

C. Fine needle aspiration

It is used to fix at a cold temperature

Acetone

Which of the following is true about formaldehyde

I. 65 RNA for formaldehyde

II. 45 DNA for formaldehyde

Both are incorrect

Decalcifying agents softens fibrous tissue

Perenyi’s fluid

Refractive index of Canada Balsam

1.524

All are aldehyde fixatives except:

A. Zenkers

B. Formol saline

C. 10% NBF

D. Formalin ammonium bromide

A. Zenkers

Substitute for XYLOL, that is non-cancerous, inexpensive

A. Clove oil

B. Coconut oil

C. Benzene

E. Aniline oil

B. Coconut oil