NON-ENZYMATIC STAINS

Stains siderotic granules (for the diagnosis of sideroblastic anemia).

PRUSSIAN BLUE STAIN

PRUSSIAN BLUE STAIN detects what in urine

Hemosiderin

PRUSSIAN BLUE STAIN is used to identify iron in what state

ferric state

PERIODIC ACID SCHIFF (PAS) stains react with what functional group

Aldehyde group

PERIODIC ACID SCHIFF (PAS) Stain reacts w/ aldehyde groups in

glycogen, mucoprotein, glycoproteins, and high molecular weight carbohydrates

Does not detect stains instead it combines with a functional group

PERIODIC ACID SCHIFF (PAS)

PERIODIC ACID SCHIFF (PAS) is Positive to all blood cells except

normal erythroblast or pronormoblast

Positive erythroblast indicates that it is affected by

AML6

Positive in erythroblasts means

Di Guglielmo disease / Erythroleukemia.

PERIODIC ACID SCHIFF (PAS) stains positive in

L1 and L2

The positivity of L1 to Pas is described to be

chunky or block-like positivity

PERIODIC ACID SCHIFF (PAS) Negative reaction

bright fuchsia pink (Pattern of staining varies with each cell type

PAS differentiates

Acute Lymphoblastic Leukemia.

Peroxidase of eosinophil is resistance to

cyanide

Used to identify peroxidase of eosinophils

Cyanide resistant peroxidase

Marker for phospholipids and lipids

SUDAN BLACK B (SBB)

SUDAN BLACK B (SBB) Has the same staining reaction as

myeloperoxidase

SBB is used to differentiates

AML from ALL

SBB Advantage over myeloperoxidase is

more stable

Sample stained in SBB can give reliable result for

months.

SBB positive reaction/color

Dark purple-black granules

SBB is More sensitive than

chloroacetate

SBB is more sensitive than chloroacetate in the demonstration of

mature & immature neutrophils and mast cells

A metachromatic stain

TOLUIDINE BLUE O

TOLUIDINE BLUE O binds with what in the blood cell

Mucopolysaccharide

TOLUIDINE BLUE O is used for the recognition of

basophils and mast cells

TOLUIDINE BLUE O positive reaction/color

Reddish-violet