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Where does light independent stage occur?
Stroma
How many times does the Calvin Cycle occur?
6 times
What are the 3 main stages of the Calvin Cycle?
Carbon Fixation
Reduction
Regeneration
Carbon Fixation
CO2 (1C) combines w. RuBP (5C), catalysed by RuBisCO to produce unstable 6C intermediate
C in CO2 has been fixed (incorporated from external environment into organic molecule)
6C breaks down forming 2 GP molecules (3C each)

Reduction
Energy from 2 ATP & 2 H from 2 red.NADP used to reduce 2 GP to 2TP (triose phosphate)
Supplied from LD reactions
Produces 2 ADP

Regeneration
5/6 of TP molecules used to regenerate RuBP
Requires energy (ATP) from LD reaction
Produces ADP

What happens to the 1/6 carbon?
Goes towards making glucose
Calvin cycle occurs 6 times, each time a carbon from TP goes towards making the glucose, other 5 regenerate RuBP

Why is RuBisCo inefficient?
Is competitively inhibited by oxygen, so a lot are needed to carry out photosynthesis effectively
Production of GP reduced
Only happens when conc. of CO2 becomes v. low
Uses of TP (triose phosphate)
Starting material for synthesis of carbohydrates, lipids & amino acids
Majority of it (5/6) recycled to regenerate RuBP so Calvin Cycle can occur