Fixation Review for Comp and Uses (Non Aq) EXAM 3

Acetone uses

Preserve enzymes, brain tissue & rabies, immunofluorescence

Alcohols components

Methanol, ethanol, isopropanol

Alcohols uses

touch preps, blood smears, cytology, gout crystals, glycogen

Alcoholic formalin components

Formaldehyde, ethanol, Ca acetate

Alcoholic formalin uses

Fatty tissues, glycogen, increased visibility of lymph nodes

Alcoholic zinc formalin components

Zinc salts, ethanol/isopropyl, formaldehyde

Alcoholic zinc formalin uses

Large fatty tissue, detect lymph nodes, glycogen, increased immunoreactivity, replace mercury fixative

Aq Zinc Formalin components

Zinc salts, formaldehyde

Aq Zinc Formalin Uses

Increased immunoreactivity, replace mercury

B5 components

Mercuric chloride, formaldehyde, sodium acetate

B5 uses *

Hematopoietic and lymphoid tissue, excellent nuclear detail *

Bouin Solution components

Aqueous picric acid, formaldehyde, acetic acid

Bouin Use

GI bx, delicate soft tissues, pretreatment for trichrome stain

Carnoy solution components

ethanol, chloroform, acetic acid

Cornoy uses

Cytology, glycogen, good nuclear detail

Clarke Fluid comp

ethanol, acetic acid

Clarke Fluid uses

Frozens, smears

Davidson comp

Formaldehyde, alcohol (any 3), acetic acid

Davidson uses

EYE specimens, increase visibility of lymph nodes, sub for Bouin

Formaldehyde-Glutaraldehyde comp

formal., glutar., Na phosphate and Na hydroxide

Form.-Glutar. Use

Dual purpose

Gendre solution comp

Alcoholic picric acid, formal., acetic acid

Like an alcoholic bouins

Gendre Solution uses

Glycogen

Helly solution comp

Mercuric chloride, potassium dichromate, formaldehyde

Helly uses

Hematopoietic and lymphoid tissues, excellent nuclear detail

Hollande solution comp

Copper acetate, Aq picric acid, formal., acetic acid

Bouin but with copper

Hollande uses

GI Bx, Preserve RBCs

Methacarn comp

Methanol, choloroform, acetic acid

Methacarn uses

cytology, glycogen

Orth solution comp

potassium dichromate, formal., sodium sulfate

Orth solution uses

Phenochromocytoma diagnosis, chromaffin rxn

Zamboni solution somp

Paraformaldehyde, (Aq) Picric acid, Na phosphate

Zamboni Uses

General purpose and EM

NOT light micro

Zenker solution comp

mercuric chloride, potassium dichromate, acetic acid

Zenker uses

Pretreatment for PTAH stain

Fixatives for IHC

Zinc formalin, 10%NBF

Fixative for enz histochem or immunofluorescence

Acetone

Fixatives for EM

glutaraldehyde, form-glutar., modified Milling formalin, osmium tetroxide, paraformaldehyde, Zamboni

Fixative for phospholipids

Calcium formalin

Fixative for simple lipids

Osmium tetroxide

Fixatives for glycogen

alcohol, alcoholic formalin, alcoholic zinc, carnoy, gendre, methacarn

Fixatives for GI bxs

Bouin, hollande

Fixative for Gout (and touch preps, smears, cytology, glycogen)

Alcohol

Fixatives for Hematopoietic and lymphoid tissue

B5, Helly, Zinc formalin

Fixatives for CNS

20% NBF, formalin ammonium bromide