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starch test
grind food sample with 2cm3 of distilled water, add a few drops of iodine
starch positive result
orange → blue/black
reducing sugar test
add benedict’s reagent with crushed food sample and heat in a water bath at 75 degrees - only works on reducing sugars
reducing sugar positive result
blue → green → yellow → red
protein test
2cm3 food solution, 2m3 biuret solution
protein positive result
blue → lilac
lipids test
2cm3 food solution + few drops of ethanol shake, + few drops of distilled water, shake
positive lipids result
colourless → white cloudy emulsion
non-reducing sugar
2cm3 food sample + 2cm3 Benedict's reagent, heat in water bath 75 degrees. does not change colour - not a reducing sugar. 2cm3 food sample to HCl in 75 degree water bath. slowly add sodium hydrogencarbonate to neutralise acid. redo Benedict’s test → brown/red
potassium iodide (iodine in it)
starch grains in a plant cell