MOLBIO LAB 4

What type of pipetting technique utilizes this type of method?

Forward Technique

What type of pipetting technique utilizes this type of method?

Reverse Technique

What type of pipetting technique utilizes this type of method?

Repetitive Pipetting Technique

What type of pipetting technique utilizes this type of method?

Heterogenous Sample Pipetting Technique

What type of sample/reagent utilizes this type of technique?

Aqueous solutions (buffers, diluted acids, and alkaline); Nucleotide solution

What type of sample/reagent utilizes this type of technique?

Highly viscous solutions

What type of sample/reagent utilizes this type of technique?

Adding reagents into tubes or wells of microplates

What type of sample/reagent utilizes this type of technique?

Blood or serum

What type of pipette uses a cushion of air between the piston and the liquid?

Air displacement pipette

Which pipette type has the piston directly contacting the liquid sample?

Positive displacement pipette

What pipette is used to repeatedly dispense liquid from a common reservoir?

Dispenser pipette

Which laboratory instrument is used to transfer very small volumes accurately in molecular biology laboratories?

Micropipette

Which pipette type is commonly used for microplate applications?

Multichannel pipette

What pipette type is used in cell and tissue culture when volumes greater than 1 mL are aspirated?

Serological pipette

Which pipette tips are most commonly used and are neither sterile nor filtered?

Multi-purpose tips

Which pipette tips prevent aerosol contamination during PCR procedures?

Aerosol barrier tips

What pipette tips have hydrophobic inner surfaces that reduce liquid retention?

Low retention tips

Which pipette tips are designed to reach the bottom of deep tubes and reagent bottles?

Extended length tips

Which special pipette tip has a distal end orifice that is about 70% larger than standard tips? This is used for genomic DNA and other fragile cellular samples

Wide orifice tips

Which pipette tips are specifically designed for loading samples into agarose or acrylamide gels?

Gel loading tips

Which pipette tips protect pipettes from strong acids, bases, and organic solvents?

Solvent safe carbon filter tips

What is the recommended maximum immersion depth of a pipette tip for accurate pipetting?

1cm

If asked in millimeters,

10mm

Which pipetting angle is recommended to minimize pipetting inaccuracies

Vertical

What part of the micropipette plunger is pressed first when aspirating liquid using the forward technique?

First stop

What part of the micropipette plunger is pressed to completely expel remaining liquid?

Second stop

Which disinfectant is commonly used at 70% concentration for cleaning pipettes

Ethanol

What contamination occurs when remnants of a previous sample mix with the next sample?

Carry-over contamination

What type of pipette tip is recommended for PCR and genetic studies?

Aerosol barrier tips

What laboratory activity involves loading colored dyes into wells of a gel plate?

Gel loading

What factor affecting pipette accuracy involves temperature differences between the pipette and liquid?

Temperature

What factor affecting pipette accuracy refers to mass per unit volume of a liquid?

Density

Which factor affecting pipette accuracy is influenced by air pressure at high elevations?

Altitude

In P10, what is the volume of this calibrated volume on the pipette

10 ul

In P10, what is the volume of this calibrated volume on the pipette

5.5 ul

In P200, what is the volume of this calibrated volume on the pipette

150 ul

In P1000, what is the volume of this calibrated volume on the pipette

500 ul