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list the disadvantages of traditional vaccines
Animal/human cell culture expensive, Low agent yields, Biosafety issues, Poor killing/attenuation of pathogen, Reversion of attenuated pathogen, Do not work for all pathogens, Shorter shelf-life, and Poor transportability
Discuss the pros and process for producing vaccinia vector vaccines.
What are personalized peptide vaccines? Explain the steps involved in developing a personalized peptide vaccine. How do we know that a PPV is working?
describe a batch large-scale fermentation process
describe a fed-batch large-scale fermentation process
describe a continuous culture large-scale fermentation process
airlift bioreactor
WAVE bioreactor
bubble bioreactor
stirred tank bioreactor
column filtration cell harvest
continuous centrifugation
basic cross-flow cell harvest