enzymes of DNA synthesis

whats the function of the helicase

unwinds parental double helix at replication forks

function of the single-stranded binding protein (SSBP)

binds and stabilizes the strand of DNA for a template

function of topoisomerase

relieves overwinding ahead of replication forks → breaks, swivels, rejoins DNA strands

function of primase

synthesizes RNA primer at 5’ end of lead strand 5’ on okazaki for lagging

function of DNA pol III

uses DNA as a template and synthesizes/makes new DNA by adding nucleotides to the primer or DNA strand

function of DNA pol I

removes the RNA primer that was placed from 5’ end and replaces them w/ DNA nucleotides added to the 3’ end

function of DNA ligase

joins the okazaki fragments on the lagging strand; leading strand joins 3’ end of DNA that replaces primer to red of leading strand

function of sliding clamp

holds DNA pol when nucleotides are added

function of DNA polymerase II

repairs