BioChem Exam 2 Review

At what wavelength do heterocyclic nucleic acid bases absorb UV?

260 nm

Which nucleic acid is shown here?

DNA

Which nucleic acid is shown here?

RNA

True or False: DNA polymerase adds nucleotides at the 3’ end

True

Who developed the chain termination method (dideoxy method)?

F. Sanger

The base-specific chemical cleavage was developed by?

Maxam and Gilbert

What is the precursor of hypoxanthine?

Adenine

What is the precursor of xanthine?

Guanine

How are hypoxanthine and xanthine made?

deamination

What is the direction and number of base pairs per turn of DNA type A?

Right-handed, 11 bp

What is the direction and number of base pairs per turn of DNA type B?

Right-handed, 10 bp

What is the direction and number of base pairs per turn of DNA type C?

Left-handed, 12 bp

Who discovered radioactivity?

Marie Curie

Who discovered A binds to T and C binds to G?

Erwin Chargaff

Fill in the blanks: 1962 Nobel Prize

___, ___, and ___ took credit for ___’s discovery of the DNA diffraction pattern or double helix.

James Watson, Francis Crick, Maurice Wilkins, Rosalind Franklin

Name the term for the self-insertion of an electron rich molecule (ex. an aromatic molecule) into the electron deficient double stranded helix.

(In other words, this describes flat, planar molecules slipping and binding in-between the stacked base pairs of the DNA double helix.)

Intercalation

Fill in the blanks: (a)

When S is ___, the equation for rate is ___ order in S

low, 1st

Fill in the blanks: (b)

When S is ___, the equation for rate is ___ order in S

high, 0