Culture Media

What organisms is Eosin Methylene Blue used to differentiate between, and what colors are shown?

Lactose fermenting (pink/red) and non-lactose fermenting (translucent) enterics

What organism does Thiosulfate-Citrate-Bile-Salt differentiate between?

Sucrose fermenting vibrio (V. cholerae) and non-sucrose fermenting vibrio species.

What organism does Cefsulodin-Irgisan Novobiocin differentiate between?

Mannitol fermenting Yersinia spp (enterocolitica) and non-mannitol fermenting Yersinia

What three media are used to detect Neisseria and Moraxella spp?

1. Thayer-Martin

2. Martin-Lewis

3. New York City

What media is used to detect Gardnerella vaginalis?

V Agar

What three organisms are detected by BCYE?

1. Nocardia

2. Francisella tularensis

3. Legionella

C. diptheriae can be grown on what three media (hint: one of them is a combo of test results and a media)?

1. Loeffler Medium

2. Brown halo on CTBA & urease +

3. ELEK plate

What is the ELEK plate used for?

Toxin detection in C. diptheriae

Enterobacteriaceae can be detected by what two media?

1. Kligler Agar

2. TSI slants

What four organisms are indicated by a K/K (red/red) TSI slant?

1. Pseudomonas

2. Eikenella

3. Moraxella

4. Campylobacter

What organisms are indicated by K/A (red/yellow) TSI slant?

1. Shigella

2. Serratia

3. Providencia

4. Yersinia

What organisms are indicated by K/A H2S (red/black) TSI slant?

1. Salmonella

2. Proteus

What organism is indicated by A/A (yellow/yellow) TSI slant?

E. coli

What organisms are indicated by A/A (gas) (yellow/yellow)?

1. Klebsiella

2. Enterobacter

What organism is BAP used for?

Campylobacter jejuni

What two media are used to detect Pseudomonas?

1. Cetrimide Agar

2. Hugh-Leifson Medium

What media is used to detect Burkholderia cepacia?

BCSA (B. Cepacia Selective Agar)

What two media are used to detect Bordatella?

1. Bordet-Gengou Charcoal

2. Regan-Lowe Charcoal

What media is used to detect Streptobacillus moniliforms?

BHI with 20% horse serum

What media is used to detect anaerobic GNR such as Bacteroides and Prevotella?

Laked blood-kanamycin-vancomycin (LKV) agar

What media is used to detect solely Bacteroides?

Bacterioides Bile Esculin

What two media are used to detect Anaerobic GPC & GPR?

PEA & CNA

What media is used to detect C. difficile?

CCFA agar

What organisms does Egg Yolk Agar differentiate between?

Fusarium nucleatum (lipase -) and Fusarium necrophorum (lipase +)

What is used to detect Chlamydia trachomatis?

Cell culture

What two stains are used to detect mycobacteria?

1. Auramine-Rhodamine fluorochrome stain

2. Ziehl-Neelsen aka Kinyoun

What is used to detect Mycobacterium tuberculosis?

Middlebrook (Cording) Broth

The modified acid-fast stain is used to detect what four organisms?

1. Nocardia

2. Cryptosporidium

3. Cyclospora

4. Sarcocystis