MOLBIO LAB 2

What are the different ways of expressing concentration of solutions?

Molarity, Molality, Normality, Percent Solution, X solution

Average molecular weight of dsDNA

660pg/pmol

Average molecular weight of ssDNA, RNA, and nucleotides

330pg/pmol

What is used in the quantitation of DNA extraction?

Spectrophotometer by UV absorbance methods

In relation to the question above, at what wavelengths is transmittance measured?

260 and 280 nanometers

For the measurement of absorbance in molecular diagnostics, the ratio should be between ______, which indicates the purity of DNA extracted.

1.8 and 2.0

At 260 nanometers, what is the conversion factor for dsDNA?

50 mcg/mL (dsDNA)

At 260 nanometers, what is the conversion factor for RNA?

40 mcg/mL (RNA)

At 260 nanometers, what is the conversion factor for ssDNA?

33 mcg/mL (ssDNA)

Piece of equipment used to sterilize materials through pressure, steam and high temperature.

Autoclave

Used to dry materials and supplies after washing and sterilization.

Drying oven

In relation to the question above, at what temperature can the equipment be set?

30 to 300 degrees Celsius

Used to accurately transfer very small volumes (micrometers) of liquid from one container to another.

Micropipette

Used to weigh materials and provides higher readability especially for materials with smaller weights.

Analytical Balance

Used to melt agarose gel in preparation for gel electrophoresis.

Microwave

Used for amplification purposes, wherein the mixture of isolated nucleic acids and master mix are placed.

Thermal Cycler

In relation to the question above, what is its common name?

PCR machine

In relation to the question above, which section is this equipment located?

Amplification detection room

What is the airflow of that section?

Negative pressure

This shows horizontal gels that run in acrylic gel boxes or baths.

Gel Electrophoresis

What is used to visualize the bands formed in electrophoresis?

Gel Documentation Viewer

In relation to the question above, what stain was most often used but avoided because of its carcinogenic properties?

Ethidium bromide

What are commercially available stains used in Gel Electrophoresis?

Gel Red, SYBR stains, Coomassie blue

This is used to provide a clean working area in the laboratory to protect workers from infectious agents.

Biosafety Cabinet

What equipment is specifically used in the molecular laboratory?

Biosafety cabinet class II

Which section is that located?

Specimen preparation room

It provides a dry area for a heating solution for incubation purposes.

Heat Block

Used to separate solid particles from a liquid suspension in DNA extraction

Mini Centrifuge

Provides absorbance readouts of solutions at specific wavelengths.

Absorbance reader with a nanodrop

It states that the absorbance of a solution is directly proportional to its concentration.

Beer-Lamberts Law

What is mainly used to check for purity of nucleic acids?

Nanodrop

Name the equipment

Microtube

Name the equipment

Spin Column