Citrate Test: does the organism produce citrate permease

If given a description of results, know what that means about the organism's ability to produce citrate permease 

• If organism grows it is positive (green and blue) 

• If there is no growth it is dead thus negative 

If given the details of an organism ability to produce citrate permease, be able to describe how the results should come out (presence//absence of growth on slant) 

• Citrate positive: blue produce citrate permease 

• Citrate positive: green slang produce citrate permease 

• Citrate negative: green does not produce citrate permease

What is the name of the enzyme we are testing for in the citrate test

Citrate permease 

You inoculate a citrate test and after incubation, you observe that the media is blue and there is growth on the slant. What does this tell you about the organism 

Positive for citrate permease

You inoculate a citrate test and after incubation, you observe that the media is green and there is no growth on the slant. What does this tell you about the organism ability to break down citrate

Negative cannot break down citrate 

You inoculate a citrate test and after incubation, you observe that the media is green and there is growth on the slant. What does this tell you about the organism?

Positive for citrate permease