Laboratory Techniques in DNA Manipulation

These flashcards cover key concepts related to laboratory techniques in DNA manipulation, including PCR, gel electrophoresis, and health and safety practices.

PCR

Polymerase Chain Reaction; a technique used to amplify specific DNA segments.

Gel electrophoresis

A method for separating DNA fragments based on their size by applying an electric field.

Recombinant DNA technology

Techniques used to manipulate DNA and generate proteins for various applications.

Ligation

The process of joining two pieces of DNA together, often involving a vector like pBluescript.

Template DNA

The original strand of DNA that serves as a template for amplification in PCR.

Restriction enzymes

Proteins that cut DNA at specific sequences, allowing for DNA manipulation.

Agarose gel

A porous medium used in gel electrophoresis to separate DNA fragments.

TAE buffer

A buffer solution used in electrophoresis to maintain pH and provide ions for the conductance of electricity.

dNTPs

Deoxyribonucleotide triphosphates; the building blocks of DNA used in PCR.

Taq DNA Polymerase

A heat-stable enzyme used to synthesize new DNA strands during PCR.

Bioinformatics

The use of computational tools to analyze and interpret biological data, such as DNA sequences.

Loading dye

A dye used to help visualize the progress of electrophoresis and keep samples at the bottom of wells.

DNA ladder

A mixture of DNA fragments of known sizes used as a reference for determining the size of PCR products.

Health and Safety

Protocols and equipment required to ensure safety while performing laboratory work.

PPE

Personal Protective Equipment; gear worn to minimize exposure to hazards in the laboratory.