Preparative Chromatography

These flashcards cover key concepts and vocabulary related to preparative chromatography, its techniques, processes, and applications.

Preparative Chromatography

A technique used to isolate and purify specific compounds from complex mixtures on a larger scale than analytical chromatography.

Pharmaceutical Development

The process of developing new drugs, ensuring their efficacy, safety, and quality during manufacturing.

High-Performance Liquid Chromatography (HPLC)

A widely used chromatographic technique known for its high resolution, accuracy, and efficiency, especially in drug purification.

Gas Chromatography (GC)

A method for separating volatile and thermally stable compounds, less common in preparative applications due to scale limitations.

Centrifugal Partition Chromatography (CPC)

A modern separation technique that eliminates the solid stationary phase to enhance recovery efficiency and reduce sample loss.

Mobile Phase

A solvent or solvent mixture used to carry and separate components through the column in chromatography.

Gradient Elution

A technique where the composition of the mobile phase is gradually changed to enhance separation efficiency.

Fraction Collector

A device used to collect eluted compounds in individual fractions from the chromatographic process.

Thin-Layer Chromatography (TLC)

An analytical technique used to identify and analyze the components of a mixture, often used during fraction analysis.

Evaporation

A process used to remove solvents from collected fractions to isolate the pure compound, often achieved through rotary evaporation.

Overloading

A major concern in preparative chromatography, occurring when sample concentration or volume exceeds the column capacity, reducing separation efficiency.

Affinity Chromatography

A highly specific method that separates compounds based on biological interactions, effective for purifying target molecules.

Lyophilization

A freeze-drying technique used to remove solvents while preserving heat-sensitive compounds.

Pre-filtration

A step in sample preparation to remove insoluble particles or impurities before chromatography.

Final Purification

The additional purification steps taken if the obtained compound does not meet the required purity standards.