MLT Blood Bank

Lewis

absorbed onto RBC surface, influenced by secretor genes, causes hemolytic tx rx, IGM class but can react on all phases

Anti-C

rarely encountered as a single Rh Ab

Anti-c

second most frequently encountered Rh Ab

Anti-E

most common Rh Ab

anti-e

rarest Rh Ab

120 days

Rh antibodies appear in serum _____ days after initial exposure.

2-7 days

Rh antibodies appear in serum ___ to ___ days after secondary exposure

I antigen

I-developed seen only in adults, I-fetal seen mostly in cord, IgM Ab, cold autoantibody

P antigen

naturally occuring IgM, --- PCH autoantibody called Donath Landsteiner Ab (IgG)

Kell

second to Rh system for HDN severity, CGD, muscular dystrophy, cardiomegaly

Duffy

IgG, shows dosage, capable of causing transfusion reactions and HDN, destroyed by enzymes

Kidd

shows marked dosage, IgG, active at 37c, enhanced by enzyme, may cause delayed hemolytic reactions

MNS

naturally occuring IgM, shows marked dosage, homozygous cells, react stronger with antisera, destroyed by enzymes, antigens have sialic acid in their structure, reactions enhanced by lowering pH

Anti-S, Anti P

rarely naturally occuring IgM

Anti-s, anti-Lub

rare antibody IgG

Anti-Lua

naturally occuring IgM, shows mixed field reaction, may show dosage

Lutheran Ab

destroyed by enzymes

HLA system

ankylosing spondylitis, useful for paternity testing, organ transplantation

Anti-K

most common Ab seen in bloodbank after ABO and Rh

Potentiator

substance that will enhance Ag-Ab reactions

PEG

removes water to concentrate Ab

weak-D and Anti-D in serum

D mosaic may produce antibodies to missing antigen parts

Anti-I

may mask other more significant Ab,

FFP

no acceptable alternative for neonates, should be compatible with patients RBC

"AB" plasma

can be given to "A, B, O", but can only recieve "AB"

"O" RBC

can only recieve "O" RBCs, no alternative

crossmatch specimen storage

7 days post transfusion, 1-6 celcius

IgG class Ab

Rh, Kell Duffy Kidd, Lutheran, MNSs, Xg (sex link)

IgM class Ab

Lewis, Lua, I, P, MN

Albumin agglutination phenomenon

serum contains anti-caprylate Ab, agglutinates all RBC in albumin stabilized with caprylate, only at 37 celcius

Reuleaux

increased serum protein, corrected by serum replacement

Adsorption

remove Ab from serum by reacting that Ab with specific Ag on RBC

Elution

removal and recovery of an Ab that is attached to RBC

Enzyme

technique to modify Ag on RBC (ficin, papain, bromelin, trypsin) - remove sialic acid from RBC membrane to enhance/destroy specific Ag

Ab destroyed by enzyme

MNS, duffy

Ab enhanced by enzyme

Rh, Kidd, Lewis, Anti-P, Anti-I

Neutralization-Inhibition

Binding Ab to specific Ag substance to inhibit reactivity of Ab

Max body temp to donate homologous blood

37.5 c, 99.5 f

Min weight for homologous blood donation

110 lbs/ 50kg

Indefinite retention

records of blood component/unit final disposition

Storage temp for RBC frozen

-65c or less

Shipment temp for RBC frozen

-40c or less

5 days

PLT shelf life

FFP/ Cryoprecipitate shelf life

1 year

FFP/Cryoprecipitate storage/shipment temp

-18c or less

RBC liquid shelf life

35-42 days

RBC liquid storage temp

1-6 celcius

RBC liquid shipment temp

1-10 celcius

RBC frozen, post thaw shelf life

14 days

PLT storage/shipment temp

20-24 celcius

200 series

CFR series that covers drugs

600 series

CFR series that covers biologics

12 month deferral

major surgery - blood products required

72 hour deferral

tooth extraction, released from penal/mental institution