Practical 2

What does the catalase test for?

The presence of catalase

Positive catalase result

Bubbles

Negative catalase result

No bubbles

Endospores

What does a blood agar show?

Whether bacteria produce hemolysin which breaks down RBC.

Beta

Complete RBC breakdown

Alpha

Partial RBC breakdown

Gamma

No RBC breakdown

What does MSA show?

Fermentation of mannitol.

What does yellow represent on MSA

Ferments mannitol

What does pink/colorless represent on MSA

Does not ferment mannitol

What does an EMB plate show?

Lactose fermentation

Green on EMB plate

Ferments lactose a lot

Purple on EMB plate

Ferments lactose a little

No shine on EMB plate

Does not ferment lactose

What does the SIM test show?

H2S production, indole production and motility

Contains nonmotile, and indole negative bacterium

Contains motile and indole positive bacterium

Contains motile, indole negative, and H2S producing bacterium

Indole positive result

Reagent turns red

What does methyl red detect?

The presence of a large concentration of acid end products

Methyl red positive reaction

Red

Methyl red negative reaction

Yellow

What does the VP test for?

Capability of organisms to produce nonacidic or neutral end products like acetoin.

Positive VP result

Red and white

Negative VP result

Green and yellow

What does the citrate utilization test for?

If an organism can use citrate as its only source of carbon

Positive citrate utilization result

Blue

Negative citrate utilization result

Green

LB (-pGLO)

Ampicillin sensitive E. coli will grow

LB + amp (-pGLO)

Ampicillin sensitive E. coli will not grow because it is sensitive to ampicillin 

LB + amp (+pGLO)

Ampicillin sensitive E. coli will grow because pGLO is present 

LB + amp + ara (+pGLO)

Ampicillin sensitive E. coli will glow because arabinose is present. 

P (plain)

Strain 1 and strain 2 grow on it, and they mate 

S (LB and streptomycin)

Strain 1 grows but not strain 2

A (LB and ampicillin)

Strain 1 does not grow but strain 2 does

N (LB and Nalidixic acid)

Strain 1 does not grow but strain 2 does

S and A (streptomycin and ampicillin)

Neither strain one or two grow, but they mate

Use of anaerobic jar, what goes in and why?

Organisms that grow in the throat (throat cultures), they add a GasPak pouch to absorb oxygen. 

High susceptibility to dental caries

Yellow, fermentation of the glucose to lactic acid by bacteria in the saliva. 

Low susceptibility dental caries

Green, less fermentation of the glucose to lactic acid by bacteria in the saliva.

Obligate aerobe

Obligate anaerobe

Facultative anaerobe

Microaerophile

Aerotolerant anaerobe

Neutrophil

Eosinophil

Basophil

Lymphocyte

Monocyte

Sickle cell anemia

Bent shape RBCs

Leukemia

Increased number of WBCs

Thrombocytosis

Increase number of platelets

Bacteremia/septicemia

Bacteria in blood

Thermal death time

The shortest length of time required to kill all test microbes at a specified temperature. First period where there is NOT growth.

UV sensitivity

The bacterium that is more sensitive will stop growth QUICKER

No ring present

Resistant

Ring present

Bacteria are susceptible to the antibiotic if within susceptibility spectrum.

Wet mount shows

Morphology and arrangement

Coagulase test

Detects the clumping factor (bound coagulase) present in the bacterial cell

Positive coagulase test

Agglutination occurs

Negative coagulase test

No agglutination occurs

What does DNAse test for?

Enzyme hydrolyzing DNA

Negative DNAse test

Clearing

Positive DNAse test

Opaque

CAMP test

Named after Chrisite Atkins Munch Peterson

Positive CAMP test

Arrowhead of hemolysis is visible, group B

Negative CAMP test

Arrowhead of hemolysis is not visible, group C/G/D