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Conservative Model
Semi-Conservative Model
After generation 1 of DNA replication, both strands had N^15
After generation 2 of DNA replication, two strands have N^15
Dispersive Model
Helicase
Breaks apart double strands by breaking hydrogen bonds
Topoisomerase
Relieves tension caused by unwinding
Primase
Adds RNA primers to DNA strand
DNA Polymerase III
Attaches DNA nucleotides
Moves in 3’ → 5’ direction
DNA Polymerase I
Replaces RNA primer with DNA nucleotides
DNA Ligase
Binds Okazaki fragments by catalyzing 3’C + Pi