ANHB2212 - Digestive System Development

Histogenesis

formation of different tissues from undifferentiated cells

All body cavities include these 2 things

• mesothelium lining

• parietal and visceral regions

Mesothelium

Single layer of squamous epithelium that produces serous fluid to facilitate low friction movement of organs

What are the embryological origins of the parietal and visceral peritoneum?

parietal = somatic lateral plate mesoderm

visceral = splanchnic lateral plate mesoderm

What does the celiac trunk supply?

foregut organs

What does the superior mesenteric artery supply?

midgut organs

What does the inferior mesenteric artery supply?

hindgut organs

What 5 organs are foregut derivatives?

• Stomach

• Liver

• Gall bladder

• Pancreas

• Proximal duodenum

What 8 organs are derivatives of the midgut?

• Distal duodenum

• Pancreas

• Jejunum

• Ileum

• Caecum

• Appendix

• Ascending colon

• Transverse colon (proximal 2/3)

What 4 organs are hindgut derivatives?

• Transverse colon (distal 1/3)

• Descending colon

• Sigmoid colon

• Anorectal canal (proximal 2/3)

What is the main arterial supply of the foregut organs?

Splenic a.

Left gastric a.

Common hepatic a.

What are the main arterial supply for the midgut organs?

Ileocolic a.

Right colic a.

Middle colic a.

What are the main arterial supply for the hindgut organs?

Left colic a.

Sigmoidal branches

Superior rectal a.

What is the main motor nerve supply for the foregut, midgut and hindgut?

foregut and midgut = vagus nerve

hindgut = Pelvic splanchnic nerves (S2-S4)

Peritoneum

continuous serous membrane lining the inner surface of the abdominal cavity

Mesentery

folds (reflections) of peritoneum that attach viscera to the body wall (ventral & dorsal)

Formation of the dorsal mesentery

1. Initially, primitive gut tube hangs from the posterior body wall by a broad bar of mesenchyme

2. Inferior to the septum transversum, the connection thins forming a membranous dorsal mesentery (= reflected peritoneum) extending from abdominal esophagus to rectum as a continuous sheet

What is the function of mesenteries?

• mobility of organs

• passage for blood vessels and nerves

Mesogastrium

relates to foregut structures

The Mesentery

suspends parts of the small intestine

Mesocolon

suspends parts of the colon

What does ventral mesentery suspend?

foregut organs

What is ventral mesentery derived from?

mesoderm of the septum transversum

What are the 3 ventral mesenteries?

• falciform ligament

• lesser omentum

• triangular ligaments

What does the dorsal mesentery suspend?

foregut, midgut and hindgut organs

What 4 foregut mesenteries are part of the dorsal mesentery?

• gastrosplenic ligament

• splenorenal ligament

• gastrocolic ligament

• greater omentum

What 4 midgut mesenteries are part of the dorsal mesentery?

• The Mesentery (Mesointestine)

• Mesoduodenum

• Mesoappendix

• Transverse mesocolon

What hindgut mesentery is part of the dorsal mesentery?

sigmoid mesocolon

Falciform ligament

between liver & ventral body wall

Lesser omentum

between liver & stomach & between liver & duodenum

Triangular ligaments

between liver & diaphragm

Gastrosplenic ligament

between stomach & spleen

Splenorenal ligament

between spleen & kidney

Gastrocolic ligament

between stomach & colon

What differentiates the intraperitoneal organs, primary retroperitoneal organs and secondary retroperitoneal organs?

Intraperitoneal organs – have a mesentery and are inside the peritoneal folds

Primary retroperitoneal organs – never had a mesentery and are behind the peritoneal

Secondary retroperitoneal organs – lose their mesentery during development

What are the 10 intraperitoneal organs?

• Stomach

• Appendix

• Liver

• Transverse colon

• Duodenum (first/superior part only)

• Small intestines (jejunum and ileum)

• Pancreas (only the tail)

• Rectum (upper third)

• Spleen

• Sigmoid colon

What are the 5 primary retroperitoneal organs?

• thoracic esophagus

• rectum

• kidneys

• adrenal glands

• bladder

What are the 4 secondary retroperitoneal organs?

• duodenum

• pancreas

• ascending colon

• descending colon

Peritoneal Zygosis

1. During development, secondary retroperitoneal organs get pressed against the posterior body wall

2. Causes adhesion of the dorsal mesentery to the peritoneum of the dorsal body wall

3. Results in “retro-peritonealisation” of the affected parts of the developing gut

What supplies the intraperitoneal and secondary retroperitoneal organs?

unpaired arteries of the vitelline system

When do the two muscle layers of the esophagus develop?

Inner circular layer – early week 5

Outer longitudinal layer – week 8

How is muscle development of the esophagus induced?

by gut endoderm

Describe the muscle of the adult esophagus

cranial (Adult ~ 2-4cm) = skeletal m.

caudal (Adult ~ 11cms) = smooth m.

middle = transitional mixed zone

What is the smooth and skeletal muscle of the esophagus derived from?

Smooth m. - splanchnic mesoderm of the gut

Skeletal m. - cranial paraxial mesoderm

How is the stomach attached to the body wall?

ventral mesentery (mesogastrium; lesser omentum) and dorsal mesentery (mesogastrium)

Development of the Stomach

1. Developing stomach becomes fusiform in shape

2. The cranial and caudal ends of the stomach originally lie in the body’s midline

3. There is a 90 degree clockwise rotation around the stomach’s longitudinal axis

4. This causes the left side to face ventrally & right side to face dorsally

5. The vagus nerves, which have travelled via the espohagus into the abdominal cavity also change position (left vagus faces ventrally; right vagus faces dorsally)

6. As this longitudinal rotation occurs, the stomach also starts to rotate around a ventro-dorsal axis

7. This causes the cranial (cardiac) part to shift slightly downward and to the left and the caudal (pyloric) part of the stomach to shift upward and right

8. Differential growth of the stomach during rotations gives rise to lesser curvature (pre-rotation = ventral surface - grows slower) and greater curvature (pre-rotation = dorsal surface - grows faster)

How are the greater and lesser omentum supplied?

coeliac truck derived arteries (greater omentum = left & right gastroepiploic arteries)

What are the 3 functions of the greater omentum?

• Fat deposition

• Immune function (areas called milky spots house macrophages & lymphocytes)

• Infection and wound isolation - may isolate areas of infection and trauma (wraps around)

How does the greater omentum function in large mammals?

In large mammals the greater omentum may act as a sling around abdominal organs – helps to prevents twisting & reduce friction during movement

Development of the Liver

1. The hepatic diverticulum arises as a ventral endodermal outgrowth from caudal part of the foregut (future duodenum)

2. All gut tube endoderm has the potential to form liver cells but this is repressed by inhibitors secreted by mesoderm, ectoderm, and the notochord

3. Liver development begins in the septum transversum due to signalling pathways between septum transversum & cardiac mesoderm

4. Liver cells colonise the septum transversum

5. Subsequent growth causes the liver to bulge caudally into the abdominal cavity

6. Liver develops within ventral mesentery

7. As the liver grows, the ventral mesentery becomes membranous and divided into 2 parts: Falciform ligament and Lesser omentum

8. Mesoderm on the liver’s surface differentiates into visceral peritoneum

9. As the liver grows the cranial surface of the liver comes in contact with the diaphragm

10. This results in a bare area between the liver and diaphragm where there is no peritoneum

11. At the borders of the bare area the peritoneum folds from diaphragm to liver that creates the ventral and dorsal coronary ligaments (peritoneal reflections)

12. These meet on the right and left sides of the bare area to form the triangular ligaments

What contains the umbilical vein?

Caudal edge of the falciform ligament contains the umbilical vein

How much body weight does the liver take up at week 10?

By week 10 of development, liver ~ 10% of the total body weight

How much body weight does the liver take up at at birth?

At birth liver ~ 5% of total body weight

What are the 2 functions of the liver?

• liver is large in utero due to its hematopoietic function (blood formation) where there are large areas between hepatic cells and blood vessels walls which produce red and white blood cells until week 8

• bile secretion to emulsify fat

Gall Bladder Development

1. As liver develops into the septum transversum, the biliary duct system forms

2. Where the connection between the hepatic diverticulum and the foregut (duodenum) narrows = the bile duct

3. A small ventral outgrowth develops in the bile duct = gallbladder and cystic duct

4. Positional change of the developing duodenum (partly due to stomach development) causes the bile duct entrance to pass behind the duodenum

When does the liver start producing bile?

week 12

What is the passage of bile?

1. gall bladder

2. hepatic + cystic ducts

3. bile duct

4. gastrointestinal tract (duodenum)

Meconium

Baby’s first stool that is dark green due to cells, protein, fats, intestinal secretions and bile

Pancreas Development

1. The pancreas forms from two buds arising from duodenal endoderm: Ventral bud – close to the gall bladder (ventral mesentery) Dorsal bud – dorsal mesentery

2. Splanchnic mesoderm gives rise to pancreatic connective tissue

3. Growth pattern of the stomach causes the duodenum to rotate to the right and become C-shaped

4. Change in duodenum position causes the ventral pancreatic bud to move dorsally (similar to the bile duct entrance position change)

5. Ventral bud is brought immediately below and behind the dorsal bud

6. The two buds fuse: ventral bud = inferior part of the pancreas head; dorsal bud = remainder

7. Rapid stomach growth & consequent rotation of the duodenum causes pancreas & most of the duodenum to become retroperitoneal

8. Duct system of the 2 buds fuse (distal dorsal pancreatic duct + ventral pancreatic duct) form main pancreatic duct

9. Proximal part of the dorsal pancreatic duct usually disappears but may persist as the accessory pancreatic duct

10. Main pancreatic duct + bile duct enters the duodenum at the major papilla and accessory duct associated with minor papilla

What forms the foregut-midgut boundary?

major papilla

What supplies the pancreas?

branches of coeliac trunk + superior mesenteric a

What is the function of the pancreas?

Digestive function = secretion of enzymes for breakdown of fats & carbohydrates

Pancreatic islets (glucose regulation) = secrete Insulin and Glucagon start to develop in month 3 - distributed throughout the pancreas

When does insulin secretion begin?

Insulin secretion begins ~ month 5 of development

Function of the spleen

• contains infection-fighting white blood cells

• controls the level of blood cells (WBCs, RBCs and platelets)

• filters the blood and removes any old or damaged RBCs

Development of the spleen

1. Spleen primordium appears in week 5 in the dorsal mesogastrium

2. Stomach rotation and rapid growth causes dorsal mesogastrium lengthening

3. Dorsal mesogastrium between spleen and dorsal midline swings left causing the spleen to connect to : the posterior body wall near the left kidney (= splenorenal ligament) the stomach (= gastrosplenic ligament)

What supplies the spleen?

the splenic artery – arises from the coeliac trunk

How is the midgut suspended?

Week 5 - midgut is suspended from the dorsal abdominal wall by a short mesentery

Development of the small intestine

1. A cecal primordium develops distinguishing the future ileum from future colon

2. Developing ileum elongates more rapidly than the abdominal cavity

3. Midgut develops the primary intestinal loop

4. Cranial arm of loop becomes most of the ileum

5. Caudal arm of loop becomes ascending and transverse colon

6. Apex of the loop is attached to the umbilicus by the vitelline duct & superior mesenteric artery = axis of the loop

7. Week 6 – abdominal cavity becomes too small to contain all of the intestinal loops (partly due to rapid expansion of the liver)

8. Pressure from rapidly developing abdominal organs, especially liver, forces the intestinal loop into the extraembryonic cavity of the umbilical cord = midgut herniation

9. As the primary intestinal loop herniates it rotates around the axis of the superior mesenteric artery by 90 degrees

10. Cranial arm of loop moves to embryo’s right side & caudal arm moves left This first rotation is complete by early week 8

11. The gut continues to differentiate: lengthening jejunum and ileum form folds called jejunal-ileal loops and cecum expands and develops the worm-like vermiform appendix

12. In week 10 the midgut herniation rapidly returns to the abdomen due to increased size of abdominal cavity relative to the other abdominal organs where growth has slowed

13. As the intestinal loop returns to the abdomen, it rotates by 180 degrees

14. Cecum ends up just inferior to the liver near the right iliac crest

What is the total rotation of the intestinal loop?

Total rotation of intestinal loop is 270o = 90 degrees (Weeks 6-8) + 180 degrees (week 10)

When do all intestines return to the abdominal cavity?

week 11

What supplies the large intestine?

Supplied by both superior & inferior mesenteric arteries

Development of the large intestine

1. After the large intestine returns to the abdominal cavity, it continues to lengthen & cecum moves in a caudal direction

2. The dorsal mesenteries of the ascending and descending colon shorten and fold. This causes: ascending & descending colon to press against the dorsal body wall and they start to adhere to the dorsal body wall and their mesentery gradually disappears due to zygosis

3. Ascending & descending colon = secondarily retroperitoneal

4. Transverse & sigmoid colon remain intraperitoneal (suspended by mesentery)

Cloaca

The cloaca is an endoderm-lined cavity covered by the cloacal membrane = endoderm & ectoderm (no mesoderm)

Development of the rectum

1. Between weeks 4-6 the cloaca is partitioned into a dorsal anorectal canal & ventral urogenital sinus due to the formation of a coronal partition of mesoderm - the urorectal septum

2. As caudal folding continues & the embryo grows, the urorectal septum moves closer to the cloacal membrane

3. cloacal membrane ruptures creating an anal opening for the hindgut (anorectal orifice) & a ventral opening for the urogenital sinus.

4. Between the two openings, the tip of the urorectal septum forms the perineal body

5. After the anorectal orifice forms, mesenchyme surrounding the anal canal proliferates

6. This mesenchyme forms a raised border adjacent to the anal opening = anal pit

Describe the development of the definitive anorectal canal

Cranial two-thirds of the definitive anorectal canal develops from distal hindgut - lined with endodermally derived epithelium

Caudal third of the definitive anorectal canal from the anal pit - lined with ectodermally derived epithelium

Pectinate line

endoderm-ectoderm junction between caudal third and cranial two thirds of the definitive anorectal canal made of mucosal folds

Blood supply above pectinate line

branches of the inferior mesenteric arteries & veins (hindgut)

Blood supply below the pectinate line

branches of the internal iliac arteries & veins