Genetics: Ch 19 DNA Technology

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Last updated 1:22 AM on 4/10/26
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16 Terms

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recombinant DNA

  • DNA from multiple sources combined

  • uses plasmids and restriction enzymes

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plasmid features

  • selectable marker (antibiotic resistance)

  • reporter gene

  • multiple cloning site (MCS)

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polymerase chain rxn (PCR)

amplifies DNA

  • denaturation

  • annealing

  • extension

uses Taq polymerase

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PCR applications

forensics, mutation detection, and gene expression (RT-PCR, qPCR)

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gel electrophoresis

separates DNA

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southern blot analysis

DNA

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northern blot analysis

RNA

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FISH analysis

fluorescent detection

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sanger sequencing

chain termination

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NGS

high-throughput sequencing

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TGS

long reads

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transgenic organism genetic engineering

added gene

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knockout genetic engineering

gene removed

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CRISPR-Cas9

  • guide RNa directs Cas9 to DNA

  • cuts DNA at specific sequence (PAM required)

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CRISPR applications

disease treatement (sickle cell) and genome editing

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risks of genetic engineering

off-target mutations